Genome location: Pf3D7_13_v3:1,789,220..1,790,757(+)
Genome classification: Core
Product Description: lipoyl synthase
SignalP Peptide: N/A
# Transmembrane Domains: 1
EC Numbers: 2.8.1.8 (Lipoyl synthase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0016992 | lipoate synthase activity |
| Process | GO:0009107 | lipoate biosynthetic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.05 | 0.97 |
| Trophozoite | not expressed | 0.10 | 0.94 |
| Schizont | possibly expressed | 0.13 | 0.91 |
| Gametocyte | expressed | 0.38 | 0.78 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1344600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.135 | MFS: -2.807 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1357500)
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_1357500)
Modification: Disrupted | RMgm-4607
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IDQ0
"Best" AlphaFill ligand hit: DTV ((2s,3s)-1,4-dimercaptobutane-2,3-diol, Local RMSD=0.44) with 5EXI (Global RMSD=2.37)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.8.1.8 | lipoyl synthase | No BRENDA inhibitors |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100980
Most Similar Human Ortholog: O43766
TM-align score: 0.79 | RMSD: 1.50
Seq Identity: 0.58 | Length: 301 / 415
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000121897 | lipoic acid synthetase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 22 | 17 | 41 |
| disruptive | 7 | 38 | 27 | 49 |
| missense | 5 | 37 | 25 | 44 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 63 | 25 | 32 |
| disruptive | 10 | 110 | 40 | 108 |
| missense | 7 | 92 | 33 | 80 |
PlasmoDB Total SNPs: 56
Non-coding: 33 | Synonymous: 14 | Nonsynonymous: 8 | Stop Codon: 1
Protein Length: 415 | Molecular Weight (kDa): 47.593
UniProt ID(s): Q8IDQ0
PDB ID(s): None
Isoelectric Point: 9.87
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR003698 | Lipoyl synthase |
| InterPro | IPR006638 | Elp3/MiaB/NifB |
| InterPro | IPR007197 | Radical SAM |
| InterPro | IPR031691 | Lipoyl synthase, N-terminal |
| PFam | PF04055 | Radical SAM |
| PFam | PF16881 | Lipoyl synthase, N-terminal |
| Superfamily | SSF102114 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 15225307 | The human malaria parasite Plasmodium falciparum has distinct organelle-specificlipoylation pathways. | Wrenger C, Muller S | 10.1111/j.1365-2958.2004.04112.x |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 35164549 | Roles of Ferredoxin-Dependent Proteins in the Apicoplast of Plasmodium falciparumParasites. | Swift RP, Rajaram K, ..., Prigge ST | 10.1128/mbio.03023-21 |