Genome location: Pf3D7_13_v3:1,809,393..1,810,615(-)
Genome classification: Core
Product Description: thioredoxin 2
SignalP Peptide: MKKYIFFFLFSFINFFFVYDVTC
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0097619 | PTEX complex |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005739 | mitochondrion |
| Component | GO:0020003 | symbiont-containing vacuole |
| Function | GO:0016671 | oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor |
| Function | GO:0005515 | protein binding |
| Function | GO:0047134 | protein-disulfide reductase (NAD(P)) activity |
| Function | GO:0015035 | protein-disulfide reductase activity |
| Process | GO:0045454 | cell redox homeostasis |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.28 | 0.86 |
| Trophozoite | expressed | 0.16 | 0.90 |
| Schizont | expressed | 0.03 | 0.98 |
| Gametocyte | expressed | 0.06 | 0.96 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1345100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS *tentative
MIS: 0.122 | MFS: -3.166 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1358000)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1358000)
Modification: Disrupted | RMgm-3683
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IDP4
"Best" AlphaFill ligand hit: DTU ((2r,3s)-1,4-dimercaptobutane-2,3-diol, Local RMSD=0.47) with 3ZZX (Global RMSD=2.95)
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 6 | 3 | 15 |
| disruptive | 4 | 4 | 1 | 22 |
| missense | 3 | 4 | 0 | 20 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 20 | 8 | 13 |
| disruptive | 7 | 17 | 19 | 38 |
| missense | 4 | 13 | 14 | 26 |
PlasmoDB Total SNPs: 54
Non-coding: 46 | Synonymous: 4 | Nonsynonymous: 4 | Stop Codon: 0
Protein Length: 157 | Molecular Weight (kDa): 18.63
UniProt ID(s): Q8IDP4
Isoelectric Point: 10.32
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR013766 | Thioredoxin domain |
| InterPro | IPR036249 | Thioredoxin-like superfamily |
| PFam | PF00085 | Thioredoxin domain |
| Superfamily | SSF52833 | Thioredoxin-like superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 16879648 | Structural and biochemical characterization of a mitochondrial peroxiredoxin fromPlasmodium falciparum. | Boucher IW, McMillan PJ, ..., Muller S | 10.1111/j.1365-2958.2006.05303.x |
| 19536257 | A newly discovered protein export machine in malaria parasites. | de Koning-Ward TF, Gilson PR, ..., Crabb BS | 10.1038/nature08104 |
| 21203490 | Compartmentation of redox metabolism in malaria parasites. | Kehr S, Sturm N, ..., Becker K | 10.1371/journal.ppat.1001242 |
| 22355694 | Structural insights into thioredoxin-2: a component of malaria parasite proteinsecretion machinery. | Sharma A, Sharma A, Dixit S, Sharma A | 10.1038/srep00179 |
| 37782422 | Physiological and genetic regulation of anhydrobiosis in yeast cells | Sek W, Kot AM, Rapoport A, Kieliszek M | 10.1007/s00203-023-03683-w |
| 37992069 | Sensing chemical-induced genotoxicity and oxidative stress via yeast-basedreporter assays using NanoLuc luciferase | Shichinohe M, Ohkawa S, Hirose Y, Eki T | 10.1371/journal.pone.0294571 |