PF3D7_1345200 (ROM6)

Genome location: Pf3D7_13_v3:1,811,227..1,813,968(-)

Genome classification: Core

Function and Localization

Product Description: rhomboid protease ROM6, putative

SignalP Peptide: N/A

# Transmembrane Domains: 2

EC Numbers: 3.4.21.105 (Rhomboid protease)

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.17	0.91
Trophozoite	expressed	0.70	0.61
Schizont	expressed	0.24	0.84
Gametocyte	expressed	0.47	0.74

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1345200

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.138 | MFS: -3.131 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1358100)

Relative Growth Rate: 0.13 ± 0.04
Confidence: 7.75

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: A0A5K1K8X1

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.4.21.105	rhomboid protease	dodecyl maltosidedichloroisocoumarin3,4-dichloroisocoumarinn-nonyl-beta-D-glucosidediisopropyl fluorophosphonate3,4-dichloro-1H-2-benzopyran-1-one7-amino-4-chloro-3-methoxyisocoumarin3-butyl-4-(pent-4-yn-1-yl)oxetan-2-one1-myristoyl-sn-glycero-3-phosphocholine1,2-dihexanoyl-sn-glycero-3-phosphocholine1,2-dimyristoyl-sn-glycero-3-phosphocholinephenyl 2-oxo-4-phenylazetidine-1-carboxylate...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_124366

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	25	21	38
disruptive	13	63	41	133
missense	8	60	38	112

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	10	51	30	59
disruptive	28	190	88	193
missense	16	146	76	127

PlasmoDB Total SNPs: 135

Non-coding: 99 | Synonymous: 23 | Nonsynonymous: 13 | Stop Codon: 0

Protein Information

Protein Length: 569 | Molecular Weight (kDa): 67.866

UniProt ID(s): A0A5K1K8X1

PDB ID(s): None

Isoelectric Point: 10.24

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR022764	Peptidase S54, rhomboid domain
PFam	PF01694	Peptidase S54, rhomboid domain
Superfamily	SSF144091	N/A