Genome location: Pf3D7_13_v3:1,822,843..1,825,456(+)
Genome classification: Core
Product Description: ubiquitin-conjugating enzyme E2
SignalP Peptide: N/A
# Transmembrane Domains: 1
EC Numbers: 6.3.2.19 (Transferred entry: 2.3.2.23, 2.3.2.27 and 6.2.1.45)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0005634 | nucleus |
| Function | GO:0016887 | ATP hydrolysis activity |
| Function | GO:0061631 | ubiquitin conjugating enzyme activity |
| Function | GO:0004842 | ubiquitin-protein transferase activity |
| Process | GO:0000209 | protein polyubiquitination |
| Process | GO:0016567 | protein ubiquitination |
| Process | GO:0010564 | regulation of cell cycle process |
| Process | GO:0030433 | ubiquitin-dependent ERAD pathway |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.04 | 0.98 |
| Trophozoite | possibly expressed | 0.30 | 0.81 |
| Schizont | possibly expressed | 0.13 | 0.90 |
| Gametocyte | possibly expressed | 0.14 | 0.91 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1345500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.119 | MFS: -2.878 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1358400)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IDP1
"Best" AlphaFill ligand hit: BU3 ((r,r)-2,3-butanediol, Local RMSD=0.20) with 1Z2U (Global RMSD=1.33)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.3.2.19 | ubiquitin-protein ligase | No BRENDA inhibitors |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
No passing variants found
PlasmoDB Total SNPs: 95
Non-coding: 85 | Synonymous: 4 | Nonsynonymous: 6 | Stop Codon: 0
Protein Length: 278 | Molecular Weight (kDa): 32.788
UniProt ID(s): Q8IDP1
PDB ID(s): 2H2Y
Isoelectric Point: 10.13
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000608 | Ubiquitin-conjugating enzyme E2 |
| InterPro | IPR016135 | Ubiquitin-conjugating enzyme/RWD-like |
| PFam | PF00179 | Ubiquitin-conjugating enzyme E2 |
| Superfamily | SSF54495 | Ubiquitin-conjugating enzyme/RWD-like |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 19502583 | An unusual ERAD-like complex is targeted to the apicoplast of Plasmodiumfalciparum. | Spork S, Hiss JA, ..., Przyborski JM | 10.1128/EC.00083-09 |
| 23785288 | An apicoplast localized ubiquitylation system is required for the import ofnuclear-encoded plastid proteins. | Agrawal S, Chung DW, ..., Le Roch KG | 10.1371/journal.ppat.1003426 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |