Genome location: Pf3D7_13_v3:1,827,863..1,830,813(-)
Genome classification: Core
Product Description: isocitrate dehydrogenase [NADP], mitochondrial
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 1.1.1.42 (Isocitrate dehydrogenase (NADP(+)))
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005739 | mitochondrion |
| Component | GO:0005634 | nucleus |
| Function | GO:0004450 | isocitrate dehydrogenase (NADP+) activity |
| Process | GO:0006739 | NADP metabolic process |
| Process | GO:0006102 | isocitrate metabolic process |
| Process | GO:0006099 | tricarboxylic acid cycle |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 0.06 | 0.97 |
| Trophozoite | expressed | 0.39 | 0.76 |
| Schizont | expressed | 0.11 | 0.92 |
| Gametocyte | expressed | 0.31 | 0.82 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1345700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.121 | MFS: -3.089 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1358600)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1358600)
Modification: Disrupted | RMgm-3688
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I6T2
"Best" AlphaFill ligand hit: NAP (nadp nicotinamide-adenine-dinucleotide phosphate, Local RMSD=0.01) with 2QFV (Global RMSD=1.95)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 1.1.1.42 | isocitrate dehydrogenase (NADP+) | ATPADPGTPGSHNAD+NADHEDTAGSSGHOClNADPHNADP+ML309... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| O75874 | Isocitrate dehydrogenase [NADP] cytoplasmic | HOGENOM, OMA, OrthoDB | US8957068, 473US8957068, 474US8957068, 1US8957068, 3... | |
| P48735 | Isocitrate dehydrogenase [NADP], mitochondrial | HOGENOM, OrthoDB | GSK321GSK321GSK321US10946023, Compound 1... |
Ortholog Group (OrthoMCL): OG6_100639
Most Similar Human Ortholog: O75874
TM-align score: 0.98 | RMSD: 1.16
Seq Identity: 0.63 | Length: 412 / 468
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000138413 | isocitrate dehydrogenase (NADP(+)) 1 |
| ENSG00000182054 | isocitrate dehydrogenase (NADP(+)) 2 |
Protein Length: 468 | Molecular Weight (kDa): 53.594
UniProt ID(s): Q8I6T2
PDB ID(s): None
Isoelectric Point: 8.72
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004790 | Isocitrate dehydrogenase NADP-dependent |
| InterPro | IPR019818 | Isocitrate/isopropylmalate dehydrogenase, conserved site |
| InterPro | IPR024084 | Isopropylmalate dehydrogenase-like domain |
| PFam | PF00180 | Isopropylmalate dehydrogenase-like domain |
| Superfamily | SSF53659 | N/A |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 23 | 15 | 34 |
| disruptive | 7 | 37 | 26 | 46 |
| missense | 7 | 37 | 26 | 46 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 53 | 14 | 31 |
| disruptive | 15 | 72 | 32 | 100 |
| missense | 15 | 64 | 26 | 67 |
PlasmoDB Total SNPs: 81
Non-coding: 56 | Synonymous: 14 | Nonsynonymous: 11 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 25843709 | Genetic investigation of tricarboxylic acid metabolism during the Plasmodiumfalciparum life cycle. | Ke H, Lewis IA, ..., Vaidya AB | 10.1016/j.celrep.2015.03.011 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |