Genome location: Pf3D7_13_v3:1,835,227..1,837,216(-)
Genome classification: Core
Product Description: kinetochore protein SPC25, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0031262 | Ndc80 complex |
| Component | GO:0005634 | nucleus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.03 | 0.99 |
| Trophozoite | expressed | 0.27 | 0.82 |
| Schizont | expressed | 0.14 | 0.90 |
| Gametocyte | expressed | 0.45 | 0.73 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1345900
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.124 | MFS: -3.059 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1358800)
RMgmDB ABS Phenotype: Not tested (Pb ortholog: PY17X_1364500)
Modification: Tagged | RMgm-5311
More info: PhenoPlasm Link
AlphaFill Uniprot ID: A0A5K1K999
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 16 | 10 | 17 |
| disruptive | 1 | 17 | 6 | 20 |
| missense | 1 | 17 | 6 | 20 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 26 | 14 | 13 |
| disruptive | 2 | 35 | 18 | 44 |
| missense | 2 | 30 | 15 | 35 |
PlasmoDB Total SNPs: 133
Non-coding: 122 | Synonymous: 11 | Nonsynonymous: 0 | Stop Codon: 0
Protein Length: 216 | Molecular Weight (kDa): 25.729
UniProt ID(s): A0A5K1K999
PDB ID(s): None
Isoelectric Point: 5.32
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR013255 | Chromosome segregation protein Spc25 |
| PFam | PF08234 | Chromosome segregation protein Spc25 |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 32501284 | Real-time dynamics of Plasmodium N DC80 reveals unusual modes of chromosomesegregation during parasite proliferation | Zeeshan M, Pandey R, ..., Tewari R | 10.1242/jcs.245753 |
| 37950911 | A role for beta-1,6- and beta-1,3-glucans in kinetochore function in Saccharomycescerevisiae | Kshirsagar R, Munhoven A, Tran Nguyen TM, Ehrenhofer-Murray AE | 10.1093/genetics/iyad195 |
| 36810679 | Methylation of CENP-A/Cse4 on arginine 143 and lysine 131 regulates kinetochorestability in yeast | Tran Nguyen TM, Munhoven A, ..., Ehrenhofer-Murray AE | 10.1093/genetics/iyad028 |