Genome location: Pf3D7_13_v3:1,875,452..1,878,087(-)
Genome classification: Core
Product Description: 6-cysteine protein P48/45
SignalP Peptide: N/A
# Transmembrane Domains: 1
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020036 | Maurer's cleft |
| Component | GO:0046658 | anchored component of plasma membrane |
| Component | GO:0009986 | cell surface |
| Component | GO:0005886 | plasma membrane |
| Function | GO:0005515 | protein binding |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.02 | 0.99 |
| Trophozoite | not expressed | 0.02 | 0.99 |
| Schizont | not expressed | 0.02 | 0.99 |
| Gametocyte | expressed | 1.31 | 0.47 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1346700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -1.876 | #Insertions: 3
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1359700)
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_1359600)
Modification: Mutated | RMgm-4522
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I6T1
"Best" AlphaFill ligand hit: UMP (2'-deoxyuridine 5'-monophosphate, Local RMSD=0.24) with 7S01 (Global RMSD=18.64)
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 20 | 14 | 32 |
| disruptive | 6 | 13 | 10 | 31 |
| missense | 6 | 13 | 10 | 30 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 36 | 21 | 34 |
| disruptive | 9 | 34 | 36 | 73 |
| missense | 8 | 27 | 32 | 57 |
PlasmoDB Total SNPs: 57
Non-coding: 43 | Synonymous: 3 | Nonsynonymous: 11 | Stop Codon: 0
Protein Length: 448 | Molecular Weight (kDa): 51.486
UniProt ID(s): A0A5K1K917, Q26010, Q26011, Q8I6T1, Q8STH6, Q8TA11, Q8TA12, Q8TA15, Q8TA16, Q8TA17, Q8TA19, Q8TA20
Isoelectric Point: 6.19
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR010884 | 6-Cysteine (6-Cys) domain |
| PFam | PF07422 | 6-Cysteine (6-Cys) domain |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 1380124 | Further characterization of interactions between gamete surface antigens ofPlasmodium falciparum. | Kumar N, Wizel B | 10.1016/0166-6851(92)90013-a |
| 11163248 | A central role for P48/45 in malaria parasite male gamete fertility. | van Dijk MR, Janse CJ, ..., Eling W | 10.1016/s0092-8674(01)00199-4 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16603573 | Identification and stoichiometry of glycosylphosphatidylinositol-anchoredmembrane proteins of the human malaria parasite Plasmodium falciparum. | Gilson PR, Nebl T, ..., Crabb BS | 10.1074/mcp.M600035-MCP200 |
| 18332422 | Correctly folded Pfs48/45 protein of Plasmodium falciparum elicits malariatransmission-blocking immunity in mice. | Outchkourov NS, Roeffen W, ..., Stunnenberg HG | https://pubmed.ncbi.nlm.nih.gov/18332422/ |
| 19623257 | A potent malaria transmission blocking vaccine based on codon harmonized fulllength Pfs48/45 expressed in Escherichia coli. | Chowdhury DR, Angov E, Kariuki T, Kumar N | 10.1371/journal.pone.0006352 |
| 21124765 | The dynamics of naturally acquired immune responses to Plasmodium falciparumsexual stage antigens Pfs230 & Pfs48/45 in a low endemic area in Tanzania. | Bousema T, Roeffen W, ..., Drakeley C | 10.1371/journal.pone.0014114 |
| 31631203 | A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum. | Das D, Krishnan SR, Roy A, Bulusu G | 10.1039/c9mo00124g |
| 30297725 | The Plasmodium falciparum male gametocyte protein P230p, a paralog of P230, isvital for ookinete formation and mosquito transmission | Marin-Mogollon C, van de Vegte-Bolmer M, ..., Khan SM | 10.1038/s41598-018-33236-x |
| 16103357 | Genetically attenuated, P36p-deficient malarial sporozoites induce protectiveimmunity and apoptosis of infected liver cells | van Dijk MR, Douradinha B, ..., Janse CJ | 10.1073/pnas.0500925102 |
| 11738705 | Comparative genomics in Plasmodium: a tool for the identification of genes andfunctional analysis | Thompson J, Janse CJ, Waters AP | 10.1016/s0166-6851(01)00377-2 |
| 35697275 | Cyclic constrained immunoreactive peptides from crucial P. falciparum proteins:potential implications in malaria diagnostics | Vashisht K, Srivastava S, ..., Pandey KC | 10.1016/j.trsl.2022.06.008 |
| 27438603 | Immunological Cross-Reactivity between Malaria Vaccine Target Antigen P48/45 inPlasmodium vivax and P. falciparum and Cross-Boosting of Immune Responses | Cao Y, Bansal GP, Merino K, Kumar N | 10.1371/journal.pone.0158212 |
| 25854886 | Hybridization and pre-zygotic reproductive barriers in Plasmodium | Ramiro RS, Khan SM, ..., Reece SE | 10.1098/rspb.2014.3027 |
| 33940529 | Structural and immunogenicity analysis of reconstructed ancestral and consensusP48/45 for cross-species anti malaria transmission-blocking vaccine | Ramanto KN, Nurdiansyah R | 10.1016/j.compbiolchem.2021.107495 |
| 29325573 | Genetic diversity of three surface protein genes in Plasmodium malariae fromthree Asian countries | Srisutham S, Saralamba N, ..., Imwong M | 10.1186/s12936-018-2176-x |
| 26627683 | Genetic diversity of transmission-blocking vaccine candidate Pvs48/45 inPlasmodium vivax populations in China | Feng H, Gupta B, ..., Cui L | 10.1186/s13071-015-1232-4 |
| 16824624 | Pfs47, paralog of the male fertility factor Pfs48/45, is a female specificsurface protein in Plasmodium falciparum | van Schaijk BC, van Dijk MR, ..., Sauerwein RW | 10.1016/j.molbiopara.2006.05.015 |
| 20386715 | Three members of the 6-cys protein family of Plasmodium play a role in gametefertility | van Dijk MR, van Schaijk BC, ..., Janse CJ | 10.1371/journal.ppat.1000853 |
| 34248932 | Immunoreactivity of Sera From Low to Moderate Malaria-Endemic Areas AgainstPlasmodium vivax rPvs48/45 Proteins Produced in Escherichia coli and ChineseHamster Ovary Systems | Arevalo-Herrera M, Miura K, ..., Herrera S | 10.3389/fimmu.2021.634738 |
| 38289124 | Transmission-reducing and -enhancing monoclonal antibodies against Plasmodiumvivax gamete surface protein Pvs48/45 | Bansal GP, Araujo MdS, ..., Kumar N | 10.1128/iai.00374-23 |
| 30181253 | Functional Conservation of P48/45 Proteins in the Transmission Stages ofPlasmodium vivax (Human Malaria Parasite) and P. berghei (Murine MalariaParasite) | Cao Y, Hart RJ, Bansal GP, Kumar N | 10.1128/mBio.01627-18 |
| 16537837 | Development and application of a positive-negative selectable marker system foruse in reverse genetics in Plasmodium | Braks JA, Franke-Fayard B, ..., Waters AP | 10.1093/nar/gnj033 |
| 30053393 | Expression of full-length Plasmodium falciparum P48/45 in P. berghei bloodstages: A method to express and evaluate vaccine antigens | Othman AS, Lin JW, ..., Khan SM | 10.1016/j.molbiopara.2018.07.009 |