Genome location: Pf3D7_13_v3:1,878,475..1,880,618(-)
Genome classification: Core
Product Description: 6-cysteine protein P47
SignalP Peptide: N/A
# Transmembrane Domains: 2
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0046658 | anchored component of plasma membrane |
| Component | GO:0009986 | cell surface |
| Function | GO:0046789 | host cell surface receptor binding |
| Process | GO:0042783 | evasion of host immune response |
| Process | GO:0044068 | modulation by symbiont of host cellular process |
| Process | GO:0052170 | suppression by symbiont of host innate immune response |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.03 | 0.99 |
| Trophozoite | not expressed | 0.05 | 0.97 |
| Schizont | not expressed | 0.03 | 0.98 |
| Gametocyte | expressed | 0.81 | 0.59 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1346800
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.288 | MFS: -2.563 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1359700)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1359700)
Modification: Disrupted | RMgm-3697
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IDN0
"Best" AlphaFill ligand hit: NAP (nadp nicotinamide-adenine-dinucleotide phosphate, Local RMSD=0.61) with 6A2O (Global RMSD=18.08)
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 13 | 12 | 18 |
| disruptive | 24 | 20 | 17 | 48 |
| missense | 24 | 20 | 16 | 46 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 26 | 11 | 27 |
| disruptive | 34 | 37 | 27 | 79 |
| missense | 33 | 33 | 26 | 66 |
PlasmoDB Total SNPs: 76
Non-coding: 44 | Synonymous: 10 | Nonsynonymous: 22 | Stop Codon: 0
Protein Length: 439 | Molecular Weight (kDa): 50.824
UniProt ID(s): A0A0S2ZYG3, A0A0S2ZYG4, A0A0S2ZYJ8, A0A0S2ZYJ9, A0A0S2ZYN4, A0A0S2ZYQ2, A0A0S2ZYQ3, A0A0S2ZZ09, A0A0S2ZZ45, Q8IDN0, Q9Y1I3
PDB ID(s): None
Isoelectric Point: 8.39
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR010884 | 6-Cysteine (6-Cys) domain |
| PFam | PF07422 | 6-Cysteine (6-Cys) domain |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 11163248 | A central role for P48/45 in malaria parasite male gamete fertility. | van Dijk MR, Janse CJ, ..., Eling W | 10.1016/s0092-8674(01)00199-4 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16603573 | Identification and stoichiometry of glycosylphosphatidylinositol-anchoredmembrane proteins of the human malaria parasite Plasmodium falciparum. | Gilson PR, Nebl T, ..., Crabb BS | 10.1074/mcp.M600035-MCP200 |
| 16824624 | Pfs47, paralog of the male fertility factor Pfs48/45, is a female specificsurface protein in Plasmodium falciparum. | van Schaijk BC, van Dijk MR, ..., Sauerwein RW | 10.1016/j.molbiopara.2006.05.015 |
| 23661646 | The human malaria parasite Pfs47 gene mediates evasion of the mosquito immunesystem. | Molina-Cruz A, Garver LS, ..., Barillas-Mury C | 10.1126/science.1235264 |
| 25552553 | Plasmodium falciparum evades mosquito immunity by disrupting JNK-mediatedapoptosis of invaded midgut cells. | Ramphul UN, Garver LS, ..., Barillas-Mury C | 10.1073/pnas.1423586112 |
| 27992481 | Molecular Analysis of Pfs47-Mediated Plasmodium Evasion of Mosquito Immunity. | Canepa GE, Molina-Cruz A, Barillas-Mury C | 10.1371/journal.pone.0168279 |
| 28729672 | Plasmodium berghei P47 is essential for ookinete protection from the Anophelesgambiae complement-like response. | Ukegbu CV, Giorgalli M, ..., Vlachou D | 10.1038/s41598-017-05917-6 |
| 31969456 | Plasmodium falciparum evades immunity of anopheline mosquitoes by interactingwith a Pfs47 midgut receptor. | Molina-Cruz A, Canepa GE, ..., Barillas-Mury C | 10.1073/pnas.1917042117 |