Genome location: Pf3D7_13_v3:1,889,277..1,891,491(-)
Genome classification: Core
Product Description: nucleoside transporter 1
SignalP Peptide: N/A
# Transmembrane Domains: 9
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020036 | Maurer's cleft |
| Component | GO:0016021 | integral component of membrane |
| Component | GO:0005887 | integral component of plasma membrane |
| Component | GO:0005634 | nucleus |
| Component | GO:0005886 | plasma membrane |
| Function | GO:0005337 | nucleoside transmembrane transporter activity |
| Function | GO:0015211 | purine nucleoside transmembrane transporter activity |
| Process | GO:0032238 | adenosine transport |
| Process | GO:0006863 | purine nucleobase transport |
| Process | GO:0015860 | purine nucleoside transmembrane transport |
| Process | GO:0009410 | response to xenobiotic stimulus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 1.31 | 0.45 |
| Trophozoite | expressed | 2.30 | 0.19 |
| Schizont | expressed | 0.29 | 0.84 |
| Gametocyte | expressed | 0.83 | 0.57 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1347200
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.122 | MFS: -3.111 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1360100)
Modification: Disrupted | RMgm-5292
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IDM6
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100863
Most Similar Human Ortholog: Q9BZD2
TM-align score: 0.80 | RMSD: 3.42
Seq Identity: 0.12 | Length: 381 / 422
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000112759 | solute carrier family 29 member 1 (Augustine blood group) |
| ENSG00000198246 | solute carrier family 29 member 3 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 14 | 10 | 24 |
| disruptive | 7 | 14 | 4 | 29 |
| missense | 7 | 14 | 3 | 26 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 35 | 18 | 27 |
| disruptive | 11 | 32 | 21 | 56 |
| missense | 9 | 27 | 18 | 41 |
PlasmoDB Total SNPs: 75
Non-coding: 48 | Synonymous: 11 | Nonsynonymous: 16 | Stop Codon: 0
Protein Length: 422 | Molecular Weight (kDa): 47.631
UniProt ID(s): Q8IDM6, Q9NIH7, Q9NIH8, Q9NIH9
PDB ID(s): 7YDQ
Isoelectric Point: 8.11
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002259 | Equilibrative nucleoside transporter |
| InterPro | IPR036259 | MFS transporter superfamily |
| PFam | N/A | N/A |
| Superfamily | SSF103473 | MFS transporter superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 10744765 | Isolation and functional characterization of the PfNT1 nucleoside transportergene from Plasmodium falciparum. | Carter NS, Ben Mamoun C, ..., Ullman B | 10.1074/jbc.275.14.10683 |
| 11682491 | Localization of the Plasmodium falciparum PfNT1 nucleoside transporter to theparasite plasma membrane. | Rager N, Mamoun CB, ..., Ullman B | 10.1074/jbc.M107037200 |
| 16629674 | Transport of nucleosides across the Plasmodium falciparum parasite plasmamembrane has characteristics of PfENT1. | Downie MJ, Saliba KJ, ..., Kirk K | 10.1111/j.1365-2958.2006.05125.x |
| 16751273 | The plasma membrane permease PfNT1 is essential for purine salvage in the humanmalaria parasite Plasmodium falciparum. | El Bissati K, Zufferey R, ..., Ben Mamoun C | 10.1073/pnas.0602590103 |
| 18639591 | Genetic evidence for the essential role of PfNT1 in the transport and utilizationof xanthine, guanine, guanosine and adenine by Plasmodium falciparum. | El Bissati K, Downie MJ, ..., Ben Mamoun C | 10.1016/j.molbiopara.2008.06.012 |
| 19818813 | Transport of purines and purine salvage pathway inhibitors by the Plasmodiumfalciparum equilibrative nucleoside transporter PfENT1. | Riegelhaupt PM, Cassera MB, ..., Akabas MH | 10.1016/j.molbiopara.2009.10.001 |
| 25602169 | Yeast-based high-throughput screen identifies Plasmodium falciparum equilibrativenucleoside transporter 1 inhibitors that kill malaria parasites. | Frame IJ, Deniskin R, ..., Akabas MH | 10.1021/cb500981y |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 30541922 | Accessibility of substituted cysteines in TM2 and TM10 transmembrane segments inthe Plasmodium falciparum equilibrative nucleoside transporter PfENT1. | Nishtala SN, Arora A, Reyes J, Akabas MH | 10.1074/jbc.RA118.006547 |
| 31373203 | Identification via a Parallel Hit Progression Strategy of Improved Small MoleculeInhibitors of the Malaria Purine Uptake Transporter that Inhibit Plasmodiumfalciparum Parasite Proliferation. | Sosa Y, Deniskin R, ..., Akabas MH | 10.1021/acsinfecdis.9b00168 |
| 31631203 | A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum. | Das D, Krishnan SR, Roy A, Bulusu G | 10.1039/c9mo00124g |