PF3D7_1347700 (ECT)

Genome location: Pf3D7_13_v3:1,907,067..1,911,493(+)

Genome classification: Core

Function and Localization

Product Description: ethanolamine-phosphate cytidylyltransferase

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.7.7.14 (Ethanolamine-phosphate cytidylyltransferase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005829	cytosol
Function	GO:0004306	ethanolamine-phosphate cytidylyltransferase activity
Process	GO:0008654	phospholipid biosynthetic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	expressed	1.35	0.45
Trophozoite	expressed	0.70	0.61
Schizont	expressed	0.58	0.65
Gametocyte	expressed	0.91	0.52

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1347700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.622 | MFS: -2.514 | #Insertions: 0

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1360500)

Relative Growth Rate: 0.57 ± 0.23
Confidence: 4.36

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1360500)

Modification: Disrupted | RMgm-3701

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IDM2

"Best" AlphaFill ligand hit: CHT (choline ion, Local RMSD=0.42) with 4ZCQ (Global RMSD=2.23)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.7.14	ethanolamine-phosphate cytidylyltransferase	CTPNEMPCMBoleatediphosphateiodoacetamidephosphocholineCDP-ethanolamine2-aminoethylphosphonate1-Aminoethylphosphonate3-Aminopropylphosphonatephosphodimethylethanolamine...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_100945

Most Similar Human Ortholog: I3L1F9

TM-align score: 0.90 | RMSD: 0.54

Seq Identity: 0.41 | Length: 34 / 573

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000185813	phosphate cytidylyltransferase 2, ethanolamine

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	32	18	34
disruptive	12	48	24	50
missense	9	38	19	41

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	13	53	25	37
disruptive	29	94	58	147
missense	16	68	44	77

PlasmoDB Total SNPs: 151

Non-coding: 134 | Synonymous: 9 | Nonsynonymous: 8 | Stop Codon: 0

Protein Information

Protein Length: 573 | Molecular Weight (kDa): 66.039

UniProt ID(s): Q8IDM2

PDB ID(s): None

Isoelectric Point: 5.55

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR004821	Cytidyltransferase-like domain
PFam	PF01467	Cytidyltransferase-like domain
Superfamily	SSF52374	N/A