PF3D7_1348300

Genome location: Pf3D7_13_v3:1,929,041..1,934,453(-)

Genome classification: Core

Function and Localization

Product Description: elongation factor Tu, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:1990904	ribonucleoprotein complex
Function	GO:0005525	GTP binding
Function	GO:0003924	GTPase activity
Function	GO:0043022	ribosome binding
Function	GO:0003746	translation elongation factor activity
Process	GO:0042256	mature ribosome assembly
Process	GO:0006414	translational elongation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.29	0.86
Trophozoite	expressed	0.38	0.77
Schizont	possibly expressed	0.08	0.95
Gametocyte	expressed	0.35	0.80

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1348300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.12 | MFS: -3.032 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1361100)

Relative Growth Rate: 0.09 ± 0.60
Confidence: 2.40

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IDL6

"Best" AlphaFill ligand hit: SO1 (sordarin, Local RMSD=0.27) with 1N0U (Global RMSD=3.36)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102502

Most Similar Human Ortholog: A0A3B3ITU1

TM-align score: 0.87 | RMSD: 1.99

Seq Identity: 0.41 | Length: 337 / 1394

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000140598	elongation factor like GTPase 1

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	19	65	41	72
disruptive	29	158	66	178
missense	17	141	57	157

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	31	135	56	95
disruptive	55	292	149	335
missense	32	248	120	261

PlasmoDB Total SNPs: 269

Non-coding: 132 | Synonymous: 92 | Nonsynonymous: 45 | Stop Codon: 0

Protein Information

Protein Length: 1394 | Molecular Weight (kDa): 163.126

UniProt ID(s): Q8IDL6

PDB ID(s): None

Isoelectric Point: 6.19

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000640	Elongation factor EFG, domain V-like
InterPro	IPR000795	Transcription factor, GTP-binding domain
InterPro	IPR005225	Small GTP-binding protein domain
InterPro	IPR009000	Translation protein, beta-barrel domain superfamily
InterPro	IPR020568	Ribosomal protein S5 domain 2-type fold
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
InterPro	IPR035647	EF-G domain III/V-like
PFam	PF00009	Transcription factor, GTP-binding domain
PFam	PF00679	Elongation factor EFG, domain V-like
Superfamily	SSF50447	Translation protein, beta-barrel domain superfamily
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase
Superfamily	SSF54211	Ribosomal protein S5 domain 2-type fold
Superfamily	SSF54980	EF-G domain III/V-like