PF3D7_1348700 (WDR16)

Genome location: Pf3D7_13_v3:1,955,254..1,958,358(-)

Genome classification: Core

Function and Localization

Product Description: WD repeat-containing protein 16, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	not expressed	0.01	1.00
Trophozoite	not expressed	0.02	0.99
Schizont	not expressed	0.03	0.98
Gametocyte	not expressed	0.40	0.80

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1348700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: 0.652 | #Insertions: 6

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1361500)

Relative Growth Rate: 0.91 ± 0.22
Confidence: 4.43

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1361500)

Modification: Disrupted | RMgm-3703

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: A0A5K1K923

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_104302

Most Similar Human Ortholog: Q8N1V2

TM-align score: 0.86 | RMSD: 3.10

Seq Identity: 0.21 | Length: 597 / 715

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000166596	cilia and flagella associated protein 52

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	3	36	19	53
disruptive	7	57	33	78
missense	7	55	33	76

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	10	69	26	50
disruptive	16	119	63	126
missense	13	111	61	105

PlasmoDB Total SNPs: 174

Non-coding: 135 | Synonymous: 28 | Nonsynonymous: 11 | Stop Codon: 0

Protein Information

Protein Length: 715 | Molecular Weight (kDa): 84.848

UniProt ID(s): A0A5K1K923

PDB ID(s): None

Isoelectric Point: 8.75

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001680	WD40 repeat
InterPro	IPR033015	Cilia- and flagella-associated protein 52
InterPro	IPR036322	WD40-repeat-containing domain superfamily
PFam	PF00400	WD40 repeat
Superfamily	SSF50978	WD40-repeat-containing domain superfamily