PF3D7_1349100

Genome location: Pf3D7_13_v3:1,969,601..1,971,078(-)

Genome classification: Core

Function and Localization

Product Description: nucleoside diphosphate hydrolase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.6.1.6 (Nucleoside diphosphate phosphatase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0016787	hydrolase activity
Process	GO:0006139	nucleobase-containing compound metabolic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.01	0.99
Trophozoite	possibly expressed	0.04	0.97
Schizont	possibly expressed	0.12	0.91
Gametocyte	possibly expressed	0.22	0.87

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1349100

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.957 | MFS: -0.916 | #Insertions: 1

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1361900)

Relative Growth Rate: 0.92 ± 0.13
Confidence: 5.40

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1361900)

Modification: Disrupted | RMgm-4582

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IDK8

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.6.1.6	nucleoside diphosphate phosphatase	ATPADPUDPAMPGDPNEMIMPEDTAGDPbetaSmenadioneGTPgammaSsuperoxide...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_111082

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	7	10	13
disruptive	4	7	2	9
missense	4	6	2	6

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	7	18	13	12
disruptive	7	18	11	25
missense	5	11	7	16

PlasmoDB Total SNPs: 79

Non-coding: 69 | Synonymous: 3 | Nonsynonymous: 7 | Stop Codon: 0

Protein Information

Protein Length: 196 | Molecular Weight (kDa): 23.263

UniProt ID(s): Q8IDK8

PDB ID(s): None

Isoelectric Point: 5.81

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000086	NUDIX hydrolase domain
InterPro	IPR015797	NUDIX hydrolase-like domain superfamily
PFam	PF00293	NUDIX hydrolase domain
Superfamily	SSF55811	NUDIX hydrolase-like domain superfamily