Genome location: Pf3D7_13_v3:1,971,557..1,974,585(-)
Genome classification: Core
Product Description: glutamate--tRNA ligase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 6.1.1.17 (Glutamate--tRNA ligase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005829 | cytosol |
| Component | GO:0005634 | nucleus |
| Function | GO:0005524 | ATP binding |
| Function | GO:0004818 | glutamate-tRNA ligase activity |
| Function | GO:0004819 | glutamine-tRNA ligase activity |
| Process | GO:0006425 | glutaminyl-tRNA aminoacylation |
| Process | GO:0006424 | glutamyl-tRNA aminoacylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 1.45 | 0.40 |
| Trophozoite | expressed | 1.42 | 0.33 |
| Schizont | expressed | 0.38 | 0.77 |
| Gametocyte | expressed | 0.77 | 0.60 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1349200
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.119 | MFS: -2.972 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1362000)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IDK7
"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.34) with 7WAK (Global RMSD=1.25)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.1.1.17 | glutamate-tRNA ligase | KClNH4ClheminGlu-AMSdiphosphateglutamol-AMPgamma-Globulinchloramphenicolglutamyl adenylateglutamyl uridylate1,10-phenanthrolineglutamyl cytidylate... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P47897 | Glutamine--tRNA ligase | Homo sapiens | OrthoDB | CHEMBL609187CHEMBL609187CHEMBL608302CHEMBL609496 |
Protein Length: 863 | Molecular Weight (kDa): 101.418
UniProt ID(s): Q8IDK7
PDB ID(s): 7WAI, 7WAJ, 7WAK, 7WAL, 7WAO
Isoelectric Point: 9.06
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000924 | Glutamyl/glutaminyl-tRNA synthetase |
| InterPro | IPR001412 | Aminoacyl-tRNA synthetase, class I, conserved site |
| InterPro | IPR004526 | Glutamyl-tRNA synthetase, archaeal/eukaryotic cytosolic |
| InterPro | IPR011035 | Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily |
| InterPro | IPR020058 | Glutamyl/glutaminyl-tRNA synthetase, class Ib, catalytic domain |
| InterPro | IPR020059 | Glutamyl/glutaminyl-tRNA synthetase, class Ib, anti-codon binding domain |
| InterPro | IPR036282 | Glutathione S-transferase, C-terminal domain superfamily |
| PFam | PF00749 | Glutamyl/glutaminyl-tRNA synthetase, class Ib, catalytic domain |
| PFam | PF03950 | Glutamyl/glutaminyl-tRNA synthetase, class Ib, anti-codon binding domain |
| Superfamily | SSF47616 | Glutathione S-transferase, C-terminal domain superfamily |
| Superfamily | SSF50715 | Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily |
| Superfamily | SSF52374 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 10 | 62 | 39 | 51 |
| disruptive | 39 | 102 | 56 | 125 |
| missense | 15 | 75 | 42 | 102 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 18 | 119 | 38 | 71 |
| disruptive | 62 | 218 | 90 | 157 |
| missense | 30 | 153 | 69 | 113 |
PlasmoDB Total SNPs: 121
Non-coding: 24 | Synonymous: 67 | Nonsynonymous: 29 | Stop Codon: 1
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 36370911 | Structural characterization of glutamyl-tRNA synthetase (GluRS) from Plasmodiumfalciparum | Sharma VK, Chhibber-Goel J, Yogavel M, Sharma A | 10.1016/j.molbiopara.2022.111530 |