Genome location: Pf3D7_13_v3:1,976,376..1,982,692(+)
Genome classification: Core
Product Description: tyrosine kinase-like protein
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.10.2 (Non-specific protein-tyrosine kinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0015630 | microtubule cytoskeleton |
| Function | GO:0004672 | protein kinase activity |
| Process | GO:0006468 | protein phosphorylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.25 | 0.88 |
| Trophozoite | possibly expressed | 0.53 | 0.71 |
| Schizont | expressed | 0.94 | 0.51 |
| Gametocyte | expressed | 0.86 | 0.54 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1349300
Resistome Missense Mutations: I600S, Y601D, N602D, N609S
Resistome Compounds with Missense Mutations: AN13762
Resistome # Samples with Disruptive Mutations: 1 (1 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.927 | MFS: -2.909 | #Insertions: 1
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1362100)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1362100)
Modification: Disrupted | RMgm-3707
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IDK6
"Best" AlphaFill ligand hit: ADN (adenosine, Local RMSD=0.39) with 6FEK (Global RMSD=3.29)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.10.2 | non-specific protein-tyrosine kinase | ADPPP1PP2T47ChkCskPIP2CMP6RNAiWASPmorinAG490... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100006
Most Similar Human Ortholog: E7ERW9
TM-align score: 0.63 | RMSD: 3.16
Seq Identity: 0.13 | Length: 64 / 1807
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000078061 | A-Raf proto-oncogene, serine/threonine kinase |
| ENSG00000104312 | receptor interacting serine/threonine kinase 2 |
| ENSG00000132155 | Raf-1 proto-oncogene, serine/threonine kinase |
| ENSG00000135341 | mitogen-activated protein kinase kinase kinase 7 |
| ENSG00000157764 | B-Raf proto-oncogene, serine/threonine kinase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 15 | 107 | 65 | 111 |
| disruptive | 112 | 223 | 127 | 292 |
| missense | 61 | 160 | 115 | 235 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 28 | 201 | 73 | 127 |
| disruptive | 157 | 416 | 234 | 380 |
| missense | 80 | 317 | 196 | 278 |
PlasmoDB Total SNPs: 292
Non-coding: 73 | Synonymous: 118 | Nonsynonymous: 101 | Stop Codon: 0
Protein Length: 1807 | Molecular Weight (kDa): 212.531
PDB ID(s): None
Isoelectric Point: 6.75
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000719 | Protein kinase domain |
| InterPro | IPR001245 | Serine-threonine/tyrosine-protein kinase, catalytic domain |
| InterPro | IPR001660 | Sterile alpha motif domain |
| InterPro | IPR008271 | Serine/threonine-protein kinase, active site |
| InterPro | IPR011009 | Protein kinase-like domain superfamily |
| InterPro | IPR013761 | Sterile alpha motif/pointed domain superfamily |
| InterPro | IPR017441 | Protein kinase, ATP binding site |
| PFam | PF07647 | Sterile alpha motif domain |
| PFam | PF07714 | Serine-threonine/tyrosine-protein kinase, catalytic domain |
| Superfamily | SSF47769 | Sterile alpha motif/pointed domain superfamily |
| Superfamily | SSF56112 | Protein kinase-like domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 20582613 | SAM domain-dependent activity of PfTKL3, an essential tyrosine kinase-like kinaseof the human malaria parasite Plasmodium falciparum. | Abdi A, Eschenlauer S, Reininger L, Doerig C | 10.1007/s00018-010-0434-3 |
| 25732065 | A genome-scale vector resource enables high-throughput reverse genetic screeningin a malaria parasite | Gomes AR, Bushell E, ..., Billker O | 10.1016/j.chom.2015.01.014 |