PF3D7_1350400

Genome location: Pf3D7_13_v3:2,012,425..2,015,228(+)

Genome classification: Core

Function and Localization

Product Description: ubiquitin-activating enzyme E1, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0031510	SUMO activating enzyme complex
Component	GO:0005737	cytoplasm
Function	GO:0019948	SUMO activating enzyme activity
Process	GO:0032446	protein modification by small protein conjugation
Process	GO:0016925	protein sumoylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.06	0.97
Trophozoite	possibly expressed	0.21	0.87
Schizont	possibly expressed	0.11	0.92
Gametocyte	possibly expressed	0.15	0.91

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1350400

Resistome Missense Mutations: T300I

Resistome Compounds with Missense Mutations: AN13762

Resistome # Samples with Disruptive Mutations: 1 (1 missense, 1 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.172 | MFS: -2.748 | #Insertions: 0

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1363200)

Relative Growth Rate: 0.74 ± 0.12
Confidence: 5.68

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1363200)

Modification: Disrupted | RMgm-3711

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C0H5H9

"Best" AlphaFill ligand hit: AMP (adenosine monophosphate, Local RMSD=1.15) with 1JWB (Global RMSD=2.61)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_533362

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	8	43	18	47
disruptive	24	81	34	114
missense	17	67	30	90

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	18	78	40	48
disruptive	52	211	89	264
missense	29	148	66	141

PlasmoDB Total SNPs: 120

Non-coding: 36 | Synonymous: 55 | Nonsynonymous: 29 | Stop Codon: 0

Protein Information

Protein Length: 706 | Molecular Weight (kDa): 85.105

UniProt ID(s): C0H5H9

PDB ID(s): None

Isoelectric Point: 9.22

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000594	THIF-type NAD/FAD binding fold
InterPro	IPR035985	Ubiquitin-activating enzyme
PFam	PF00899	THIF-type NAD/FAD binding fold
Superfamily	SSF69572	Ubiquitin-activating enzyme