PF3D7_1350700

Genome location: Pf3D7_13_v3:2,023,039..2,025,270(+)

Genome classification: Core

Function and Localization

Product Description: N6-adenine-specific methylase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.1.1.62 (mRNA (2'-O-methyladenosine-N(6)-)-methyltransferase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0020011	apicoplast

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	possibly expressed	0.05	0.98
Trophozoite	expressed	0.36	0.78
Schizont	expressed	0.08	0.94
Gametocyte	expressed	0.18	0.89

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1350700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.133 | MFS: -3.034 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: A0A5K1K930

"Best" AlphaFill ligand hit: SFG (sinefungin, Local RMSD=0.73) with 6M1C (Global RMSD=1.10)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.1.1.62	mRNA (2'-O-methyladenosine-N6-)-methyltransferase	N-[[3-(4-methylphenyl)adamant-1-yl]carbamoyl]-L-phenylalanine

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_106216

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	2	14	16	32
disruptive	14	49	25	68
missense	13	47	24	63

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	3	49	15	30
disruptive	23	104	62	103
missense	20	93	52	74

PlasmoDB Total SNPs: 153

Non-coding: 69 | Synonymous: 39 | Nonsynonymous: 45 | Stop Codon: 0

Protein Information

Protein Length: 561 | Molecular Weight (kDa): 66.042

UniProt ID(s): A0A5K1K930

PDB ID(s): None

Isoelectric Point: 9.87

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR029063	S-adenosyl-L-methionine-dependent methyltransferase
PFam	PF03602	N/A
Superfamily	SSF53335	S-adenosyl-L-methionine-dependent methyltransferase