Genome location: Pf3D7_13_v3:2,101,043..2,109,710(+)
Genome classification: Core
Product Description: intron-binding protein aquarius, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.1.15 (Nucleoside-triphosphate phosphatase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0071013 | catalytic step 2 spliceosome |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005634 | nucleus |
| Function | GO:0003723 | RNA binding |
| Function | GO:0003724 | RNA helicase activity |
| Process | GO:0000184 | nuclear-transcribed mRNA catabolic process, nonsense-mediated decay |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.95 | 0.58 |
| Trophozoite | expressed | 1.27 | 0.38 |
| Schizont | not expressed | 0.37 | 0.79 |
| Gametocyte | not expressed | 0.69 | 0.64 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1352700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.123 | MFS: -2.897 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IDH3
"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.06) with 5EAX (Global RMSD=4.89)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.1.15 | nucleoside-triphosphate phosphatase | ATPADPNaFRNAEDTAdATPdTTPdCTPdGTPHOClrNTPMgCl2... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102954
Most Similar Human Ortholog: O60306
TM-align score: 0.84 | RMSD: 3.33
Seq Identity: 0.30 | Length: 1317 / 2533
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000021776 | aquarius intron-binding spliceosomal factor |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 22 | 128 | 78 | 142 |
| disruptive | 73 | 344 | 235 | 465 |
| missense | 50 | 307 | 209 | 413 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 48 | 253 | 98 | 171 |
| disruptive | 139 | 754 | 352 | 654 |
| missense | 90 | 633 | 305 | 472 |
PlasmoDB Total SNPs: 251
Non-coding: 45 | Synonymous: 122 | Nonsynonymous: 84 | Stop Codon: 0
Protein Length: 2533 | Molecular Weight (kDa): 304.306
UniProt ID(s): Q8IDH3
PDB ID(s): None
Isoelectric Point: 6.62
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR026300 | CWF11 family |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR032174 | Intron-binding protein aquarius, N-terminal |
| InterPro | IPR041677 | DNA2/NAM7 helicase, helicase domain |
| InterPro | IPR041679 | DNA2/NAM7 helicase-like, C-terminal |
| PFam | PF13086 | DNA2/NAM7 helicase, helicase domain |
| PFam | PF13087 | DNA2/NAM7 helicase-like, C-terminal |
| PFam | PF16399 | Intron-binding protein aquarius, N-terminal |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 37398028 | The debranching enzyme Dbr1 regulates lariat turnover and intron splicing | Buerer L, Clark NE, ..., Fairbrother WG | 10.21203/rs.3.rs-2931976/v1 |
| 34986795 | Caenorhabditis elegans ETR-1/CELF has broad effects on the muscle celltranscriptome, including genes that regulate translation and neuroblastmigration | Ochs ME, McWhirter RM, ..., Lundquist EA | 10.1186/s12864-021-08217-6 |