Genome location: Pf3D7_13_v3:2,143,723..2,145,387(+)
Genome classification: Core
Product Description: RNA polymerase II transcription factor B subunit 4, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.7.6 (DNA-directed RNA polymerase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0000439 | transcription factor TFIIH core complex |
| Component | GO:0005675 | transcription factor TFIIH holo complex |
| Process | GO:0006289 | nucleotide-excision repair |
| Process | GO:0070816 | phosphorylation of RNA polymerase II C-terminal domain |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.06 | 0.97 |
| Trophozoite | expressed | 0.21 | 0.86 |
| Schizont | not expressed | 0.03 | 0.98 |
| Gametocyte | not expressed | 0.12 | 0.93 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1353500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.12 | MFS: -2.95 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1130100)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IDG5
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.7.6 | DNA-directed RNA polymerase | BRENDA SOAP query unsuccessful |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_103072
Most Similar Human Ortholog: A0A087WYD5
TM-align score: 0.76 | RMSD: 2.33
Seq Identity: 0.32 | Length: 142 / 326
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000111358 | general transcription factor IIH subunit 3 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 21 | 16 | 27 |
| disruptive | 4 | 12 | 14 | 41 |
| missense | 3 | 11 | 13 | 39 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 45 | 16 | 31 |
| disruptive | 7 | 42 | 52 | 78 |
| missense | 3 | 37 | 44 | 54 |
PlasmoDB Total SNPs: 66
Non-coding: 51 | Synonymous: 13 | Nonsynonymous: 2 | Stop Codon: 0
Protein Length: 326 | Molecular Weight (kDa): 38.319
UniProt ID(s): Q8IDG5
PDB ID(s): None
Isoelectric Point: 7.74
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004600 | TFIIH subunit Tfb4/GTF2H3 |
| PFam | PF03850 | TFIIH subunit Tfb4/GTF2H3 |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 10506223 | The TFB4 subunit of yeast TFIIH is required for both nucleotide excision repairand RNA polymerase II transcription | Feaver WJ, Huang W, Friedberg EC | 10.1074/jbc.274.41.29564 |
| 27381459 | Function of Conserved Topological Regions within the Saccharomyces cerevisiaeBasal Transcription Factor TFIIH | Warfield L, Luo J, Ranish J, Hahn S | 10.1128/MCB.00182-16 |
| 14500720 | Revised subunit structure of yeast transcription factor IIH (TFIIH) andreconciliation with human TFIIH | Takagi Y, Komori H, ..., Kornberg RD | 10.1074/jbc.C300417200 |
| 10681587 | Subunit interactions in yeast transcription/repair factor TFIIH. Requirement forTfb3 subunit in nucleotide excision repair | Feaver WJ, Huang W, ..., Friedberg EC | 10.1074/jbc.275.8.5941 |
| 9235928 | Genes for Tfb2, Tfb3, and Tfb4 subunits of yeast transcription/repair factor IIH.Homology to human cyclin-dependent kinase activating kinase and IIH subunits | Feaver WJ, Henry NL, ..., Kornberg RD | 10.1074/jbc.272.31.19319 |