PF3D7_1353800

Genome location: Pf3D7_13_v3:2,151,014..2,152,684(-)

Genome classification: Core

Function and Localization

Product Description: proteasome subunit alpha type-4, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.4.25.1 (Proteasome endopeptidase complex)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Component	GO:0005634	nucleus
Component	GO:0005839	proteasome core complex
Component	GO:0019773	proteasome core complex, alpha-subunit complex
Function	GO:0004175	endopeptidase activity
Process	GO:0010498	proteasomal protein catabolic process
Process	GO:0010499	proteasomal ubiquitin-independent protein catabolic process
Process	GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process
Process	GO:0006511	ubiquitin-dependent protein catabolic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.09	0.95
Trophozoite	expressed	0.44	0.73
Schizont	expressed	0.14	0.90
Gametocyte	expressed	0.18	0.89

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1353800

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.13 | MFS: -3.135 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IDG3

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.4.25.1	proteasome endopeptidase complex	ATPSDSPSIEDTANH4+EGTATLCKALLNNMDANLVSPI 1PA28...

Orthology to BindingDB Entries:

UniProt	Protein Name	Homology Source	BindingDB Ligands
P23639	Proteasome subunit alpha type-2	OrthoDB	Carmaphycin A (1)(2S)-N-[(4S)-2,6-dimethyl-3-oxohept-1-en-4-yl]-2-[(2S)-2-hexanamido-3-methylbutanamido]-N',N'-dimethylpentanediamide (4)(2S)-N-[(4S)-2,6-dimethyl-3-oxohept-1-en-4-yl]-2-[(2S)-2-hexanamido-3-methylbutanamido]-N',N'-dimethylpentanediamide (4)Carmaphycin A (1)...
P60900	Proteasome subunit alpha type-6	OrthoDB	cid_66069med.21724, Compound 15CPZ analog, 2CPZ analog, 5...
P25786	Proteasome subunit alpha type-1	OrthoDB	CHEMBL4872258CHEMBL4877708

Orthology Information

Ortholog Group (OrthoMCL): OG6_101968

Most Similar Human Ortholog: H0YKT8

TM-align score: 0.96 | RMSD: 1.33

Seq Identity: 0.51 | Length: 180 / 246

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000041357	proteasome 20S subunit alpha 4

Protein Information

Protein Length: 246 | Molecular Weight (kDa): 27.948

UniProt ID(s): Q8IDG3

PDB ID(s): 5FMG

Isoelectric Point: 5.88

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000426	Proteasome alpha-subunit, N-terminal domain
InterPro	IPR001353	Proteasome, subunit alpha/beta
InterPro	IPR029055	Nucleophile aminohydrolases, N-terminal
PFam	PF00227	Proteasome, subunit alpha/beta
PFam	PF10584	Proteasome alpha-subunit, N-terminal domain
Superfamily	SSF56235	Nucleophile aminohydrolases, N-terminal

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	2	19	5	14
disruptive	0	5	4	18
missense	0	5	4	18

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	6	23	16	15
disruptive	0	15	19	63
missense	0	10	18	50

PlasmoDB Total SNPs: 127

Non-coding: 116 | Synonymous: 8 | Nonsynonymous: 3 | Stop Codon: 0