PF3D7_1353900

Genome location: Pf3D7_13_v3:2,153,175..2,155,528(+)

Genome classification: Core

Function and Localization

Product Description: proteasome subunit alpha type-7, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.4.25.1 (Proteasome endopeptidase complex)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Component	GO:0005634	nucleus
Component	GO:0005839	proteasome core complex
Component	GO:0019773	proteasome core complex, alpha-subunit complex
Function	GO:0004175	endopeptidase activity
Process	GO:0010498	proteasomal protein catabolic process
Process	GO:0010499	proteasomal ubiquitin-independent protein catabolic process
Process	GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process
Process	GO:0006511	ubiquitin-dependent protein catabolic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	expressed	0.13	0.94
Trophozoite	expressed	0.55	0.68
Schizont	expressed	0.16	0.89
Gametocyte	expressed	0.18	0.89

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1353900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.167 | MFS: -2.941 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IDG2

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.4.25.1	proteasome endopeptidase complex	ATPSDSPSIEDTANH4+EGTATLCKALLNNMDANLVSPI 1PA28...

Orthology to BindingDB Entries:

UniProt	Protein Name	Homology Source	BindingDB Ligands
P23639	Proteasome subunit alpha type-2	OrthoDB	Carmaphycin A (1)(2S)-N-[(4S)-2,6-dimethyl-3-oxohept-1-en-4-yl]-2-[(2S)-2-hexanamido-3-methylbutanamido]-N',N'-dimethylpentanediamide (4)(2S)-N-[(4S)-2,6-dimethyl-3-oxohept-1-en-4-yl]-2-[(2S)-2-hexanamido-3-methylbutanamido]-N',N'-dimethylpentanediamide (4)Carmaphycin A (1)...
P60900	Proteasome subunit alpha type-6	OrthoDB	cid_66069med.21724, Compound 15CPZ analog, 2CPZ analog, 5...
P25786	Proteasome subunit alpha type-1	OrthoDB	CHEMBL4872258CHEMBL4877708

Orthology Information

Ortholog Group (OrthoMCL): OG6_101207

Most Similar Human Ortholog: O14818

TM-align score: 0.96 | RMSD: 1.34

Seq Identity: 0.48 | Length: 241 / 241

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000101182	proteasome 20S subunit alpha 7
ENSG00000154611	proteasome 20S subunit alpha 8

Protein Information

Protein Length: 241 | Molecular Weight (kDa): 27.228

UniProt ID(s): Q8IDG2

PDB ID(s): 5FMG

Isoelectric Point: 6.49

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000426	Proteasome alpha-subunit, N-terminal domain
InterPro	IPR001353	Proteasome, subunit alpha/beta
InterPro	IPR029055	Nucleophile aminohydrolases, N-terminal
PFam	PF00227	Proteasome, subunit alpha/beta
PFam	PF10584	Proteasome alpha-subunit, N-terminal domain
Superfamily	SSF56235	Nucleophile aminohydrolases, N-terminal

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	16	4	20
disruptive	2	5	2	21
missense	2	4	2	13

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	5	30	13	16
disruptive	4	23	19	60
missense	2	15	13	32

PlasmoDB Total SNPs: 96

Non-coding: 91 | Synonymous: 4 | Nonsynonymous: 1 | Stop Codon: 0

PMID	Title	Authors	DOI/Link
23181666	Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum.	Oehring SC, Woodcroft BJ, ..., Voss TS	10.1186/gb-2012-13-11-r108
31384003	The structure of the PA28-20S proteasome complex from Plasmodium falciparum andimplications for proteostasis.	Xie SC, Metcalfe RD, ..., Tilley L	10.1038/s41564-019-0524-4