Genome location: Pf3D7_13_v3:2,159,676..2,163,263(-)
Genome classification: Core
Product Description: phosphoinositide phosphatase SAC1
SignalP Peptide: N/A
# Transmembrane Domains: 3
EC Numbers: 3.1.3.56 (Inositol-polyphosphate 5-phosphatase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005794 | Golgi apparatus |
| Component | GO:0005783 | endoplasmic reticulum |
| Function | GO:0043813 | phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity |
| Function | GO:0004438 | phosphatidylinositol-3-phosphatase activity |
| Function | GO:0043812 | phosphatidylinositol-4-phosphate phosphatase activity |
| Process | GO:0046856 | phosphatidylinositol dephosphorylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.11 | 0.94 |
| Trophozoite | expressed | 0.28 | 0.83 |
| Schizont | expressed | 0.51 | 0.70 |
| Gametocyte | expressed | 0.57 | 0.68 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1354200
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -1.869 | #Insertions: 4
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IDF9
"Best" AlphaFill ligand hit: OMP (orotidine-5'-monophosphate, Local RMSD=1.66) with 2ZA1 (Global RMSD=15.93)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.3.56 | inositol-polyphosphate 5-phosphatase | ATPADPAMPSDSEDTACdCl2vanadatephosphateDisulfiramthimerosalTriton X-100Phenylglyoxal... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101364
Most Similar Human Ortholog: F8WDN7
TM-align score: 0.89 | RMSD: 2.45
Seq Identity: 0.28 | Length: 280 / 803
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000211456 | SAC1 like phosphatidylinositide phosphatase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 11 | 38 | 27 | 65 |
| disruptive | 20 | 112 | 67 | 125 |
| missense | 16 | 95 | 62 | 115 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 15 | 87 | 37 | 65 |
| disruptive | 39 | 233 | 100 | 201 |
| missense | 30 | 202 | 77 | 139 |
PlasmoDB Total SNPs: 194
Non-coding: 114 | Synonymous: 53 | Nonsynonymous: 27 | Stop Codon: 0
Protein Length: 803 | Molecular Weight (kDa): 95.045
UniProt ID(s): Q8IDF9
PDB ID(s): None
Isoelectric Point: 9
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002013 | SAC domain |
| PFam | PF02383 | SAC domain |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 28983103 | Characterization of a putative Plasmodium falciparum SAC1phosphoinositide-phosphatase homologue potentially required for survival duringthe asexual erythrocytic stages. | Theriault C, Richard D | 10.1038/s41598-017-12762-0 |
| 35640825 | Essential roles of phosphatidylinositol 4-phosphate phosphatases Sac1p and Sjl3pin yeast autophagosome formation | Muramoto M, Yamakuchi Y, ..., Fujita A | 10.1016/j.bbalip.2022.159184 |
| 37507017 | Increased Phospholipid Flux Bypasses Overlapping Essential Requirements for theYeast Sac1p Phosphoinositide Phosphatase and ER-PM Membrane Contact Sites | Nenadic A, Zaman MF, ..., Beh CT | 10.1016/j.jbc.2023.105092 |
| 38258560 | Effect of ATG8 or SAC1 deficiency on the cell proliferation and lifespan of thelong-lived PMT1 deficiency yeast cells | Cui H, Cui X, ..., Liu X | 10.1093/femsle/fnad121 |