Product Description: large subunit rRNA methyltransferase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.1.1.- (Methyltransferases.)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0036396 | RNA N6-methyladenosine methyltransferase complex |
| Component | GO:0005730 | nucleolus |
| Component | GO:0030687 | preribosome, large subunit precursor |
| Function | GO:0008173 | RNA methyltransferase activity |
| Function | GO:0016435 | rRNA (guanine) methyltransferase activity |
| Function | GO:0008650 | rRNA (uridine-2'-O-)-methyltransferase activity |
| Process | GO:0001510 | RNA methylation |
| Process | GO:0000466 | maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) |
| Process | GO:0000463 | maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) |
| Process | GO:0031167 | rRNA methylation |
| Process | GO:0006364 | rRNA processing |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 1.52 | 0.38 |
| Trophozoite | expressed | 0.95 | 0.53 |
| Schizont | not expressed | 0.24 | 0.87 |
| Gametocyte | possibly expressed | 0.73 | 0.61 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1354300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.133 | MFS: -2.886 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1130900)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IDF8
"Best" AlphaFill ligand hit: SAM (s-adenosylmethionine, Local RMSD=0.61) with 7R7O (Global RMSD=1.26)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.1.1.- | Ex: homocysteine S-methyltransferase | EDTACTABNickelA9145CtylosintacrineTween 80FTI-2628macrocinadenosineRelomycinphosphate... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101655
Most Similar Human Ortholog: J3KS36
TM-align score: 0.91 | RMSD: 1.65
Seq Identity: 0.52 | Length: 145 / 1019
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000108592 | FtsJ RNA 2-O-methyltransferase 3 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 13 | 80 | 23 | 77 |
| disruptive | 24 | 163 | 92 | 168 |
| missense | 12 | 140 | 83 | 154 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 28 | 126 | 40 | 76 |
| disruptive | 66 | 298 | 116 | 232 |
| missense | 30 | 252 | 106 | 188 |
PlasmoDB Total SNPs: 206
Non-coding: 91 | Synonymous: 66 | Nonsynonymous: 47 | Stop Codon: 2
Protein Length: 1019 | Molecular Weight (kDa): 120.251
UniProt ID(s): Q8IDF8
PDB ID(s): None
Isoelectric Point: 9.56
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002877 | Ribosomal RNA methyltransferase FtsJ domain |
| InterPro | IPR012920 | Ribosomal RNA methyltransferase, Spb1, C-terminal |
| InterPro | IPR015507 | Ribosomal RNA large subunit methyltransferase E |
| InterPro | IPR024576 | Ribosomal RNA methyltransferase Spb1, domain of unknown function DUF3381 |
| InterPro | IPR028589 | AdoMet-dependent rRNA methyltransferase Spb1-like |
| InterPro | IPR029063 | S-adenosyl-L-methionine-dependent methyltransferase |
| PFam | PF01728 | Ribosomal RNA methyltransferase FtsJ domain |
| PFam | PF07780 | Ribosomal RNA methyltransferase, Spb1, C-terminal |
| PFam | PF11861 | Ribosomal RNA methyltransferase Spb1, domain of unknown function DUF3381 |
| Superfamily | SSF53335 | S-adenosyl-L-methionine-dependent methyltransferase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 31384004 | Transcriptome-wide dynamics of extensive m(6)A mRNA methylation during Plasmodiumfalciparum blood-stage development. | Baumgarten S, Bryant JM, ..., Scherf A | 10.1038/s41564-019-0521-7 |