Genome location: Pf3D7_13_v3:2,173,757..2,176,285(+)
Genome classification: Core
Product Description: adenylosuccinate synthetase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 6.3.4.4 (Adenylosuccinate synthase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Function | GO:0005525 | GTP binding |
| Function | GO:0004019 | adenylosuccinate synthase activity |
| Process | GO:0044208 | 'de novo' AMP biosynthetic process |
| Process | GO:0006167 | AMP biosynthetic process |
| Process | GO:0046040 | IMP metabolic process |
| Process | GO:0006164 | purine nucleotide biosynthetic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.34 | 0.84 |
| Trophozoite | expressed | 0.79 | 0.57 |
| Schizont | expressed | 0.09 | 0.94 |
| Gametocyte | expressed | 0.21 | 0.88 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1354500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.122 | MFS: -3.107 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q9U8D3
"Best" AlphaFill ligand hit: IMO (6-o-phosphoryl inosine monophosphate, Local RMSD=0.30) with 1P9B (Global RMSD=0.60)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.3.4.4 | adenylosuccinate synthase | ATPADPUDPAMPGDPKClCMPUMPGMPCDPIMPIDP... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P30520 | Adenylosuccinate synthetase isozyme 2 | Homo sapiens | OMA, OrthoDB | PhosphohydantocidinCHEMBL118357HADACIDIN |
Ortholog Group (OrthoMCL): OG6_100659
Most Similar Human Ortholog: G3V5D8
TM-align score: 0.94 | RMSD: 0.71
Seq Identity: 0.32 | Length: 63 / 442
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000035687 | adenylosuccinate synthase 2 |
| ENSG00000185100 | adenylosuccinate synthase 1 |
Protein Length: 442 | Molecular Weight (kDa): 50.065
PDB ID(s): None
Isoelectric Point: 7.61
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001114 | Adenylosuccinate synthetase |
| InterPro | IPR018220 | Adenylosuccinate synthase, GTP-binding site |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR033128 | Adenylosuccinate synthase, active site |
| PFam | PF00709 | Adenylosuccinate synthetase |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 27 | 13 | 34 |
| disruptive | 2 | 27 | 11 | 32 |
| missense | 2 | 26 | 11 | 25 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 11 | 47 | 19 | 36 |
| disruptive | 5 | 65 | 29 | 83 |
| missense | 5 | 54 | 21 | 51 |
PlasmoDB Total SNPs: 159
Non-coding: 139 | Synonymous: 15 | Nonsynonymous: 5 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 15500910 | Unique kinetic mechanism of Plasmodium falciparum adenylosuccinate synthetase. | Raman J, Mehrotra S, Anand RP, Balaram H | 10.1016/j.molbiopara.2004.06.013 |
| 22289630 | Mutational analysis of cysteine 328 and cysteine 368 at the interface ofPlasmodium falciparum adenylosuccinate synthetase. | Mehrotra S, B Ningappa M, ..., Balaram H | 10.1016/j.bbapap.2012.01.007 |
| 38353487 | Behenic Acid as a multi-target inhibiting antibacterial phytochemical againstVibrio parahaemolyticus and Aeromonas hydrophila for effective management ofaquaculture infections: an in-silico, in-vitro & in-vivo experimentation | Ravi L, Kumar K A, ..., Jain P | 10.1080/07391102.2024.2317988 |