Genome location: Pf3D7_13_v3:2,180,143..2,183,414(-)
Genome classification: Core
Product Description: metacaspase-1
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.4.22.- (Cysteine endopeptidases.)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Function | GO:0004198 | calcium-dependent cysteine-type endopeptidase activity |
| Function | GO:0008233 | peptidase activity |
| Process | GO:0006915 | apoptotic process |
| Process | GO:0006508 | proteolysis |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | possibly expressed | 0.06 | 0.97 |
| Trophozoite | not expressed | 0.10 | 0.94 |
| Schizont | possibly expressed | 0.07 | 0.95 |
| Gametocyte | expressed | 0.32 | 0.81 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1354800
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -2.117 | #Insertions: 2
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1302300)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1131400)
Modification: Disrupted | RMgm-3094
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IDF3
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.4.22.- | Ex: staphylococcal cysteine proteinase | NEME64SpiSDSCysEDTAPCMBEGTAPMSFTLCKTPCKMENT... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 35 | 17 | 37 |
| disruptive | 8 | 84 | 45 | 105 |
| missense | 8 | 83 | 45 | 103 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 13 | 63 | 36 | 33 |
| disruptive | 21 | 156 | 84 | 158 |
| missense | 21 | 148 | 72 | 117 |
PlasmoDB Total SNPs: 108
Non-coding: 52 | Synonymous: 42 | Nonsynonymous: 14 | Stop Codon: 0
Protein Length: 613 | Molecular Weight (kDa): 71.69
PDB ID(s): None
Isoelectric Point: 8.66
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR029030 | Caspase-like domain superfamily |
| PFam | PF00656 | N/A |
| Superfamily | SSF49562 | Caspase-like domain superfamily |
| Superfamily | SSF52129 |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 21858231 | Plasmodium falciparum metacaspase PfMCA-1 triggers a z-VAD-fmk inhibitableprotease to promote cell death. | Meslin B, Beavogui AH, Fasel N, Picot S | 10.1371/journal.pone.0023867 |
| 37977356 | Metacaspase (Pf MCA-1) as antimalarial drug target: An in silico approach andtheir biological validation | Yadav K, Kuldeep J, ..., Tripathi R | 10.1016/j.lfs.2023.122271 |
| 34384491 | Requirement of Toxoplasma gondii metacaspases for IMC1 maturation, endodyogenyand virulence in mice | Li M, Liu J, ..., Liu Q | 10.1186/s13071-021-04878-0 |
| 37052630 | Tfs1, transcription elongation factor TFIIS, has an impact on chromosomesegregation affected by pka1 deletion in Schizosaccharomyces pombe | Takenaka K, Nishioka S, ..., Matsuo Y | 10.1007/s00294-023-01268-0 |
| 37002543 | Yca1 metacaspase: diverse functions determine how yeast live and let die | Lam DK, Sherlock G | 10.1093/femsyr/foad022 |