Genome location: Pf3D7_13_v3:2,186,494..2,190,804(+)
Genome classification: Core
Product Description: DNA replication licensing factor MCM6
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.4.12 (DNA helicase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0042555 | MCM complex |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005634 | nucleus |
| Function | GO:0008094 | ATP-dependent activity, acting on DNA |
| Function | GO:0003677 | DNA binding |
| Function | GO:0003688 | DNA replication origin binding |
| Function | GO:0005515 | protein binding |
| Function | GO:0003697 | single-stranded DNA binding |
| Process | GO:0006270 | DNA replication initiation |
| Process | GO:0006268 | DNA unwinding involved in DNA replication |
| Process | GO:0000727 | double-strand break repair via break-induced replication |
| Process | GO:1902969 | mitotic DNA replication |
| Process | GO:0006267 | pre-replicative complex assembly involved in nuclear cell cycle DNA replication |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.10 | 0.95 |
| Trophozoite | expressed | 0.89 | 0.53 |
| Schizont | expressed | 0.51 | 0.70 |
| Gametocyte | expressed | 0.63 | 0.65 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1355100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.122 | MFS: -3.094 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1131600)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IDF0
"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.14) with 4R7Z (Global RMSD=5.38)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.4.12 | DNA helicase | No BRENDA inhibitors |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101983
Most Similar Human Ortholog: Q14566
TM-align score: 0.81 | RMSD: 3.90
Seq Identity: 0.36 | Length: 731 / 929
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000076003 | minichromosome maintenance complex component 6 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 31 | 25 | 71 |
| disruptive | 3 | 12 | 19 | 47 |
| missense | 2 | 10 | 16 | 43 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 12 | 100 | 37 | 55 |
| disruptive | 5 | 50 | 32 | 89 |
| missense | 3 | 42 | 25 | 74 |
PlasmoDB Total SNPs: 158
Non-coding: 136 | Synonymous: 18 | Nonsynonymous: 4 | Stop Codon: 0
Protein Length: 929 | Molecular Weight (kDa): 105.674
UniProt ID(s): Q8IDF0
PDB ID(s): None
Isoelectric Point: 4.91
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001208 | MCM domain |
| InterPro | IPR008049 | DNA replication licensing factor Mcm6 |
| InterPro | IPR012340 | Nucleic acid-binding, OB-fold |
| InterPro | IPR018525 | Mini-chromosome maintenance, conserved site |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR031327 | Mini-chromosome maintenance protein |
| InterPro | IPR033762 | MCM OB domain |
| InterPro | IPR041024 | Mcm6, C-terminal winged-helix domain |
| InterPro | IPR041562 | MCM, AAA-lid domain |
| PFam | PF00493 | MCM domain |
| PFam | PF17207 | MCM OB domain |
| PFam | PF17855 | MCM, AAA-lid domain |
| PFam | PF18263 | Mcm6, C-terminal winged-helix domain |
| Superfamily | SSF50249 | Nucleic acid-binding, OB-fold |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 16257456 | Pre-replication complex organization in the atypical DNA replication cycle ofPlasmodium falciparum: characterization of the mini-chromosome maintenance (MCM)complex formation. | Patterson S, Robert C, ..., Chakrabarti D | 10.1016/j.molbiopara.2005.09.006 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 37482133 | Genome-wide binding sites of Plasmodium falciparum mini chromosome maintenanceprotein MCM6 show new insights into parasite DNA replication | Shekhar S, Verma S, ..., Dhar SK | 10.1016/j.bbamcr.2023.119546 |