Genome location: Pf3D7_13_v3:2,198,698..2,202,351(+)
Genome classification: Core
Product Description: serine/threonine protein phosphatase 5
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.1.3.16 (Protein-serine/threonine phosphatase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005829 | cytosol |
| Component | GO:0005634 | nucleus |
| Function | GO:0016791 | phosphatase activity |
| Function | GO:0004722 | protein serine/threonine phosphatase activity |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 0.27 | 0.87 |
| Trophozoite | expressed | 0.56 | 0.67 |
| Schizont | expressed | 0.09 | 0.94 |
| Gametocyte | expressed | 0.46 | 0.74 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1355500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.898 | MFS: -2.949 | #Insertions: 1
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1131900)
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_1131900)
Modification: Tagged | RMgm-4641
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IDE7
"Best" AlphaFill ligand hit: NHE (2-[n-cyclohexylamino]ethane sulfonic acid, Local RMSD=0.06) with 6GHM (Global RMSD=2.03)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.3.16 | protein-serine/threonine phosphatase | ATPADPUDPAMPGTPUTPUMPNaFEDTAEGTAcAMPNADPH... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102598
Most Similar Human Ortholog: P53041
TM-align score: 0.94 | RMSD: 1.91
Seq Identity: 0.43 | Length: 485 / 658
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000011485 | protein phosphatase 5 catalytic subunit |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 33 | 19 | 39 |
| disruptive | 12 | 62 | 46 | 110 |
| missense | 11 | 59 | 44 | 101 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 11 | 87 | 25 | 48 |
| disruptive | 23 | 171 | 68 | 153 |
| missense | 18 | 146 | 61 | 93 |
PlasmoDB Total SNPs: 138
Non-coding: 99 | Synonymous: 24 | Nonsynonymous: 15 | Stop Codon: 0
Protein Length: 658 | Molecular Weight (kDa): 76.889
UniProt ID(s): Q8IDE7, Q8WQR3, Q962N7
PDB ID(s): None
Isoelectric Point: 4.81
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004843 | Calcineurin-like phosphoesterase domain, ApaH type |
| InterPro | IPR006186 | Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase |
| InterPro | IPR011236 | Serine/threonine protein phosphatase 5 |
| InterPro | IPR011990 | Tetratricopeptide-like helical domain superfamily |
| InterPro | IPR013235 | PPP domain |
| InterPro | IPR019734 | Tetratricopeptide repeat |
| PFam | PF00149 | Calcineurin-like phosphoesterase domain, ApaH type |
| PFam | PF08321 | PPP domain |
| PFam | PF13181 | Tetratricopeptide repeat |
| Superfamily | SSF48452 | Tetratricopeptide-like helical domain superfamily |
| Superfamily | SSF56300 |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 25011111 | Genome-wide functional analysis of Plasmodium protein phosphatases reveals keyregulators of parasite development and differentiation. | Guttery DS, Poulin B, ..., Tewari R | 10.1016/j.chom.2014.05.020 |
| 31202684 | Plasmodium berghei serine/threonine protein phosphatase PP5 plays a critical rolein male gamete fertility. | Zhu X, Sun L, ..., Cui L | 10.1016/j.ijpara.2019.03.007 |