Genome location: Pf3D7_13_v3:2,205,950..2,212,554(-)
Genome classification: Core
Product Description: NLI interacting factor-like phosphatase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.1.3.16 (Protein-serine/threonine phosphatase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005634 | nucleus |
| Function | GO:0008420 | RNA polymerase II CTD heptapeptide repeat phosphatase activity |
| Process | GO:0070940 | dephosphorylation of RNA polymerase II C-terminal domain |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 0.79 | 0.63 |
| Trophozoite | expressed | 0.44 | 0.74 |
| Schizont | expressed | 0.53 | 0.68 |
| Gametocyte | expressed | 0.60 | 0.68 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1355700
Resistome Missense Mutations: P605S, E1194K
Resistome Compounds with Missense Mutations: Desoxyepothilone B, MMV665794
Resistome # Samples with Disruptive Mutations: 4 (4 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.143 | MFS: -2.928 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1132000)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1132000)
Modification: Tagged | RMgm-1090
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IDE5
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.3.16 | protein-serine/threonine phosphatase | ATPADPUDPAMPGTPUTPUMPNaFEDTAEGTAcAMPNADPH... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 58 | 43 | 93 |
| disruptive | 30 | 197 | 104 | 219 |
| missense | 20 | 177 | 93 | 193 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 17 | 147 | 54 | 100 |
| disruptive | 66 | 480 | 188 | 398 |
| missense | 42 | 398 | 155 | 247 |
PlasmoDB Total SNPs: 266
Non-coding: 172 | Synonymous: 54 | Nonsynonymous: 40 | Stop Codon: 0
Protein Length: 1288 | Molecular Weight (kDa): 151.546
UniProt ID(s): Q8IDE5
PDB ID(s): None
Isoelectric Point: 6.85
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004274 | FCP1 homology domain |
| InterPro | IPR036412 | HAD-like superfamily |
| InterPro | IPR036420 | BRCT domain superfamily |
| InterPro | IPR039189 | CTD phosphatase Fcp1 |
| PFam | PF03031 | FCP1 homology domain |
| Superfamily | SSF52113 | BRCT domain superfamily |
| Superfamily | SSF56784 | HAD-like superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 25011111 | Genome-wide functional analysis of Plasmodium protein phosphatases reveals keyregulators of parasite development and differentiation. | Guttery DS, Poulin B, ..., Tewari R | 10.1016/j.chom.2014.05.020 |