PF3D7_1355900

Genome location: Pf3D7_13_v3:2,216,285..2,218,436(+)

Genome classification: Core

Function and Localization

Product Description: RWD domain-containing protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	expressed	0.12	0.94
Trophozoite	expressed	0.19	0.87
Schizont	expressed	0.03	0.98
Gametocyte	expressed	0.36	0.79

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1355900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: 0.991 | #Insertions: 2

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IDE3

"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=8.09) with 4KXF (Global RMSD=13.47)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_103700

Most Similar Human Ortholog: Q9UIY3

TM-align score: 0.44 | RMSD: 3.89

Seq Identity: 0.14 | Length: 155 / 405

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000013392	RWD domain containing 2A
ENSG00000156253	RWD domain containing 2B

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	2	39	24	39
disruptive	7	58	37	110
missense	6	58	35	93

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	8	63	26	40
disruptive	20	139	66	122
missense	17	124	59	89

PlasmoDB Total SNPs: 75

Non-coding: 40 | Synonymous: 19 | Nonsynonymous: 16 | Stop Codon: 0

Protein Information

Protein Length: 405 | Molecular Weight (kDa): 47.316

UniProt ID(s): Q8IDE3

PDB ID(s): None

Isoelectric Point: 5.92

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR006575	RWD domain
InterPro	IPR010541	Domain of unknown function DUF1115
InterPro	IPR016135	Ubiquitin-conjugating enzyme/RWD-like
InterPro	IPR017359	Uncharacterised conserved protein UCP038021, RWD
PFam	PF05773	RWD domain
PFam	PF06544	Domain of unknown function DUF1115
Superfamily	SSF54495	Ubiquitin-conjugating enzyme/RWD-like