PF3D7_1356400

Genome location: Pf3D7_13_v3:2,234,959..2,238,932(+)

Genome classification: Core

Function and Localization

Product Description: phosphatase 2A regulatory subunit-related protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.1.3.16 (Protein-serine/threonine phosphatase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0000159	protein phosphatase type 2A complex
Function	GO:0019888	protein phosphatase regulator activity
Process	GO:0006470	protein dephosphorylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.03	0.99
Trophozoite	not expressed	0.29	0.81
Schizont	not expressed	0.13	0.91
Gametocyte	not expressed	0.30	0.82

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1356400

Resistome Missense Mutations: F567C

Resistome Compounds with Missense Mutations: Desoxyepothilone B

Resistome # Samples with Disruptive Mutations: 1 (1 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.16 | MFS: -2.804 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1132700)

Relative Growth Rate: 0.21 ± 0.44
Confidence: 3.02

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IDD8

"Best" AlphaFill ligand hit: ORO (orotic acid, Local RMSD=0.85) with 1TV5 (Global RMSD=28.14)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.1.3.16	protein-serine/threonine phosphatase	ATPADPUDPAMPGTPUTPUMPNaFEDTAEGTAcAMPNADPH...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101579

Most Similar Human Ortholog: Q9Y5P8

TM-align score: 0.64 | RMSD: 4.27

Seq Identity: 0.24 | Length: 427 / 1006

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000073711	protein phosphatase 2 regulatory subunit Balpha
ENSG00000167393	protein phosphatase 2 regulatory subunit Bbeta
ENSG00000273850	protein phosphatase 2 regulatory subunit Bbeta
ENSG00000276438	protein phosphatase 2 regulatory subunit Bbeta

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	11	64	45	84
disruptive	18	130	90	181
missense	18	124	85	168

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	22	133	47	80
disruptive	37	303	135	265
missense	33	272	118	171

PlasmoDB Total SNPs: 160

Non-coding: 85 | Synonymous: 46 | Nonsynonymous: 29 | Stop Codon: 0

Protein Information

Protein Length: 1006 | Molecular Weight (kDa): 120.58

UniProt ID(s): Q8IDD8

PDB ID(s): None

Isoelectric Point: 4.8

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR011992	EF-hand domain pair
InterPro	IPR041534	PP2A regulatory subunit B'', EF-hand domain
PFam	PF17958	PP2A regulatory subunit B'', EF-hand domain
Superfamily	SSF47473	EF-hand domain pair