About Gene List

PF3D7_1357200 (GluRS)

Genome location: Pf3D7_13_v3:2,271,135..2,274,371(+)

Genome classification: Core

Function and Localization

Product Description: glutamate--tRNA ligase

SignalP Peptide: MIFIYFFFVTLIIINLNTIES

# Transmembrane Domains: 1

EC Numbers: 6.1.1.17 (Glutamate--tRNA ligase)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0020011 apicoplast
Function GO:0004818 glutamate-tRNA ligase activity
Function GO:0004819 glutamine-tRNA ligase activity
Process GO:0006424 glutamyl-tRNA aminoacylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite not expressed N/A N/A
Ring not expressed 0.11 0.95
Trophozoite possibly expressed 0.34 0.78
Schizont not expressed 0.08 0.94
Gametocyte expressed 0.24 0.86

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1357200

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.182 | MFS: -3.308 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IDD3

"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.46) with 7WAK (Global RMSD=2.39)

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
6.1.1.17 glutamate-tRNA ligase KClNH4ClheminGlu-AMSdiphosphateglutamol-AMPgamma-Globulinchloramphenicolglutamyl adenylateglutamyl uridylate1,10-phenanthrolineglutamyl cytidylate...

Orthology to BindingDB Entries:

UniProt Protein Name Species Homology Source BindingDB Ligands
Q9CXJ1 Probable glutamate--tRNA ligase, mitochondrial Mus musculus OrthoDB, OrthoMCL BLAST Glu-AMS
P04805 Glutamate--tRNA ligase Escherichia coli OrthoMCL, OrthoMCL BLAST Glu-AMS

Orthology Information

Ortholog Group (OrthoMCL): OG6_101161

Most Similar Human Ortholog: Q5JPH6

TM-align score: 0.77 | RMSD: 4.21

Seq Identity: 0.28 | Length: 481 / 574

All Human Orthologs (OrthoMCL):

Gene ID Description
ENSG00000103356 glutamyl-tRNA synthetase 2, mitochondrial

Protein Information

Protein Length: 574 | Molecular Weight (kDa): 68.368

UniProt ID(s): Q8IDD3

PDB ID(s): None

Isoelectric Point: 9.38

Protein Domain Annotations:

Source Family ID Description
InterPro IPR000924 Glutamyl/glutaminyl-tRNA synthetase
InterPro IPR001412 Aminoacyl-tRNA synthetase, class I, conserved site
InterPro IPR004527 Glutamate-tRNA ligase, bacterial/mitochondrial
InterPro IPR008925 Aminoacyl-tRNA synthetase, class I, anticodon-binding
InterPro IPR020058 Glutamyl/glutaminyl-tRNA synthetase, class Ib, catalytic domain
InterPro IPR033910 Glutamyl-tRNA synthetase
PFam PF00749 Glutamyl/glutaminyl-tRNA synthetase, class Ib, catalytic domain
Superfamily SSF48163 Aminoacyl-tRNA synthetase, class I, anticodon-binding
Superfamily SSF52374

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 6 31 26 37
disruptive 8 79 40 92
missense 8 77 39 89

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 11 58 28 44
disruptive 18 144 76 119
missense 12 136 73 92

PlasmoDB Total SNPs: 177

Non-coding: 141 | Synonymous: 23 | Nonsynonymous: 13 | Stop Codon: 0

Associated Publications

PMID Title Authors DOI/Link
16267556 A protein interaction network of the malaria parasite Plasmodium falciparum. LaCount DJ, Vignali M, ..., Hughes RE 10.1038/nature04104
24072705 A nondiscriminating glutamyl-tRNA synthetase in the plasmodium apicoplast: thefirst enzyme in an indirect aminoacylation pathway. Mailu BM, Ramasamay G, ..., Gardner MJ 10.1074/jbc.M113.507467
30212465 Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. Boucher MJ, Ghosh S, ..., Yeh E 10.1371/journal.pbio.2005895
36370911 Structural characterization of glutamyl-tRNA synthetase (GluRS) from Plasmodiumfalciparum Sharma VK, Chhibber-Goel J, Yogavel M, Sharma A 10.1016/j.molbiopara.2022.111530