Genome location: Pf3D7_13_v3:2,271,135..2,274,371(+)
Genome classification: Core
Product Description: glutamate--tRNA ligase
SignalP Peptide: MIFIYFFFVTLIIINLNTIES
# Transmembrane Domains: 1
EC Numbers: 6.1.1.17 (Glutamate--tRNA ligase)
Curated GO (PlasmoDB):
Type | GO Term | Name |
---|---|---|
Component | GO:0020011 | apicoplast |
Function | GO:0004818 | glutamate-tRNA ligase activity |
Function | GO:0004819 | glutamine-tRNA ligase activity |
Process | GO:0006424 | glutamyl-tRNA aminoacylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
Stage | LR class | MCA mean | MCA prop. zeros |
---|---|---|---|
Sporozoite | not expressed | N/A | N/A |
Ring | not expressed | 0.11 | 0.95 |
Trophozoite | possibly expressed | 0.34 | 0.78 |
Schizont | not expressed | 0.08 | 0.94 |
Gametocyte | expressed | 0.24 | 0.86 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1357200
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.182 | MFS: -3.308 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IDD3
"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.46) with 7WAK (Global RMSD=2.39)
BRENDA EC Inhibitors:
EC # | Name | EC Inhibitors |
---|---|---|
6.1.1.17 | glutamate-tRNA ligase | KClNH4ClheminGlu-AMSdiphosphateglutamol-AMPgamma-Globulinchloramphenicolglutamyl adenylateglutamyl uridylate1,10-phenanthrolineglutamyl cytidylate... |
Orthology to BindingDB Entries:
Ortholog Group (OrthoMCL): OG6_101161
Most Similar Human Ortholog: Q5JPH6
TM-align score: 0.77 | RMSD: 4.21
Seq Identity: 0.28 | Length: 481 / 574
All Human Orthologs (OrthoMCL):
Gene ID | Description |
---|---|
ENSG00000103356 | glutamyl-tRNA synthetase 2, mitochondrial |
Protein Length: 574 | Molecular Weight (kDa): 68.368
UniProt ID(s): Q8IDD3
PDB ID(s): None
Isoelectric Point: 9.38
Protein Domain Annotations:
Source | Family ID | Description |
---|---|---|
InterPro | IPR000924 | Glutamyl/glutaminyl-tRNA synthetase |
InterPro | IPR001412 | Aminoacyl-tRNA synthetase, class I, conserved site |
InterPro | IPR004527 | Glutamate-tRNA ligase, bacterial/mitochondrial |
InterPro | IPR008925 | Aminoacyl-tRNA synthetase, class I, anticodon-binding |
InterPro | IPR020058 | Glutamyl/glutaminyl-tRNA synthetase, class Ib, catalytic domain |
InterPro | IPR033910 | Glutamyl-tRNA synthetase |
PFam | PF00749 | Glutamyl/glutaminyl-tRNA synthetase, class Ib, catalytic domain |
Superfamily | SSF48163 | Aminoacyl-tRNA synthetase, class I, anticodon-binding |
Superfamily | SSF52374 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
variant type | common | rare | doubleton | singleton |
---|---|---|---|---|
synonymous | 6 | 31 | 26 | 37 |
disruptive | 8 | 79 | 40 | 92 |
missense | 8 | 77 | 39 | 89 |
Any inclusion in genotype call
variant type | common | rare | doubleton | singleton |
---|---|---|---|---|
synonymous | 11 | 58 | 28 | 44 |
disruptive | 18 | 144 | 76 | 119 |
missense | 12 | 136 | 73 | 92 |
PlasmoDB Total SNPs: 177
Non-coding: 141 | Synonymous: 23 | Nonsynonymous: 13 | Stop Codon: 0
PMID | Title | Authors | DOI/Link |
---|---|---|---|
16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
24072705 | A nondiscriminating glutamyl-tRNA synthetase in the plasmodium apicoplast: thefirst enzyme in an indirect aminoacylation pathway. | Mailu BM, Ramasamay G, ..., Gardner MJ | 10.1074/jbc.M113.507467 |
30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
36370911 | Structural characterization of glutamyl-tRNA synthetase (GluRS) from Plasmodiumfalciparum | Sharma VK, Chhibber-Goel J, Yogavel M, Sharma A | 10.1016/j.molbiopara.2022.111530 |