PF3D7_1357900

Genome location: Pf3D7_13_v3:2,295,079..2,297,485(+)

Genome classification: Core

Function and Localization

Product Description: pyrroline-5-carboxylate reductase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 1.5.1.2 (Pyrroline-5-carboxylate reductase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005634	nucleus
Function	GO:0004735	pyrroline-5-carboxylate reductase activity
Process	GO:0055129	L-proline biosynthetic process
Process	GO:0006561	proline biosynthetic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.07	0.96
Trophozoite	expressed	0.22	0.87
Schizont	expressed	0.01	0.99
Gametocyte	expressed	0.06	0.97

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1357900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.585 | MFS: -2.388 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1134200)

Relative Growth Rate: 0.94 ± 0.12
Confidence: 5.59

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1134200)

Modification: Disrupted | RMgm-3108

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IDC6

"Best" AlphaFill ligand hit: MPO (3[n-morpholino]propane sulfonic acid, Local RMSD=0.23) with 5BSE (Global RMSD=2.62)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
1.5.1.2	pyrroline-5-carboxylate reductase	ATPADPGTPCTPKClSDSNAD+NADHEDTANADPHNADP+NH2OH...

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
P32322	Pyrroline-5-carboxylate reductase 1, mitochondrial		OrthoDB, OrthoMCL BLAST	CHEMBL4462797CHEMBL4442920CHEMBL4452615

Orthology Information

Ortholog Group (OrthoMCL): OG6_100382

Most Similar Human Ortholog: Q96C36

TM-align score: 0.93 | RMSD: 1.54

Seq Identity: 0.34 | Length: 259 / 262

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000104524	pyrroline-5-carboxylate reductase 3
ENSG00000143811	pyrroline-5-carboxylate reductase 2
ENSG00000183010	pyrroline-5-carboxylate reductase 1
ENSG00000276657	pyrroline-5-carboxylate reductase 3

Protein Information

Protein Length: 262 | Molecular Weight (kDa): 28.379

UniProt ID(s): Q27722, Q8IDC6

PDB ID(s): 2RCY

Isoelectric Point: 8.07

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000304	Pyrroline-5-carboxylate reductase
InterPro	IPR008927	6-phosphogluconate dehydrogenase-like, C-terminal domain superfamily
InterPro	IPR028939	Pyrroline-5-carboxylate reductase, catalytic, N-terminal
InterPro	IPR029036	Pyrroline-5-carboxylate reductase, dimerisation domain
InterPro	IPR036291	NAD(P)-binding domain superfamily
PFam	PF03807	Pyrroline-5-carboxylate reductase, catalytic, N-terminal
PFam	PF14748	Pyrroline-5-carboxylate reductase, dimerisation domain
Superfamily	SSF48179	6-phosphogluconate dehydrogenase-like, C-terminal domain superfamily
Superfamily	SSF51735	NAD(P)-binding domain superfamily

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	20	8	15
disruptive	3	24	21	44
missense	3	24	21	38

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	8	34	8	15
disruptive	6	73	31	66
missense	5	66	29	43

PlasmoDB Total SNPs: 88

Non-coding: 75 | Synonymous: 7 | Nonsynonymous: 6 | Stop Codon: 0