Product Description: patatin-like phospholipase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 1
EC Numbers: 3.1.1.4 (Phospholipase A(2))
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Function | GO:0016298 | lipase activity |
| Process | GO:0006629 | lipid metabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.14 | 0.93 |
| Trophozoite | not expressed | 0.67 | 0.64 |
| Schizont | possibly expressed | 0.48 | 0.74 |
| Gametocyte | not expressed | 0.63 | 0.66 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1358000
Resistome Missense Mutations: S317R
Resistome Compounds with Missense Mutations: AN13762
Resistome # Samples with Disruptive Mutations: 1 (1 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.551 | MFS: -3.029 | #Insertions: 1
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1134300)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: A0A5K1K9G0
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.1.4 | phospholipase A2 | BRENDA SOAP query unsuccessful |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_103723
Most Similar Human Ortholog: A0A0C4DG51
TM-align score: 0.56 | RMSD: 5.29
Seq Identity: 0.24 | Length: 436 / 2012
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000135241 | patatin like phospholipase domain containing 8 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 22 | 103 | 69 | 125 |
| disruptive | 71 | 327 | 188 | 388 |
| missense | 48 | 298 | 174 | 345 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 33 | 213 | 90 | 133 |
| disruptive | 127 | 632 | 297 | 514 |
| missense | 88 | 545 | 247 | 401 |
PlasmoDB Total SNPs: 338
Non-coding: 137 | Synonymous: 108 | Nonsynonymous: 92 | Stop Codon: 1
Protein Length: 2012 | Molecular Weight (kDa): 238.206
UniProt ID(s): A0A5K1K9G0
PDB ID(s): None
Isoelectric Point: 8.14
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002641 | Patatin-like phospholipase domain |
| InterPro | IPR016035 | Acyl transferase/acyl hydrolase/lysophospholipase |
| PFam | PF01734 | Patatin-like phospholipase domain |
| Superfamily | SSF52151 | Acyl transferase/acyl hydrolase/lysophospholipase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 28419631 | TgPL2, a patatin-like phospholipase domain-containing protein, is involved in themaintenance of apicoplast lipids homeostasis in Toxoplasma. | Leveque MF, Berry L, ..., Besteiro S | 10.1111/mmi.13694 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 31772212 | Functional annotation of serine hydrolases in the asexual erythrocytic stage ofPlasmodium falciparum. | Elahi R, Ray WK, ..., Klemba M | 10.1038/s41598-019-54009-0 |