Genome location: Pf3D7_13_v3:2,314,206..2,316,111(-)
Genome classification: Core
Product Description: rhomboid protease ROM7, putative
SignalP Peptide: N/A
# Transmembrane Domains: 6
EC Numbers: 3.4.21.105 (Rhomboid protease)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0005739 | mitochondrion |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.03 | 0.98 |
| Trophozoite | not expressed | 0.20 | 0.86 |
| Schizont | possibly expressed | 0.19 | 0.86 |
| Gametocyte | not expressed | 0.37 | 0.78 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1358300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.174 | MFS: -3.438 | #Insertions: 1
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: A0A5K1K991
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.4.21.105 | rhomboid protease | dodecyl maltosidedichloroisocoumarin3,4-dichloroisocoumarinn-nonyl-beta-D-glucosidediisopropyl fluorophosphonate3,4-dichloro-1H-2-benzopyran-1-one7-amino-4-chloro-3-methoxyisocoumarin3-butyl-4-(pent-4-yn-1-yl)oxetan-2-one1-myristoyl-sn-glycero-3-phosphocholine1,2-dihexanoyl-sn-glycero-3-phosphocholine1,2-dimyristoyl-sn-glycero-3-phosphocholinephenyl 2-oxo-4-phenylazetidine-1-carboxylate... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 15 | 9 | 24 |
| disruptive | 3 | 29 | 19 | 62 |
| missense | 3 | 29 | 19 | 56 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 32 | 14 | 24 |
| disruptive | 9 | 80 | 46 | 89 |
| missense | 6 | 61 | 42 | 59 |
PlasmoDB Total SNPs: 61
Non-coding: 47 | Synonymous: 10 | Nonsynonymous: 4 | Stop Codon: 0
Protein Length: 340 | Molecular Weight (kDa): 41.012
UniProt ID(s): A0A5K1K991
PDB ID(s): None
Isoelectric Point: 10.08
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR022764 | Peptidase S54, rhomboid domain |
| PFam | PF01694 | Peptidase S54, rhomboid domain |
| Superfamily | SSF144091 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |
| 8754840 | ROM7/BEM4 encodes a novel protein that interacts with the Rho1p small GTP-bindingprotein in Saccharomyces cerevisiae | Hirano H, Tanaka K, ..., Takai Y | 10.1128/MCB.16.8.4396 |