PF3D7_1359100

Genome location: Pf3D7_13_v3:2,347,380..2,350,101(-)

Genome classification: Core

Function and Localization

Product Description: riboflavin kinase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.7.1.26 (Riboflavin kinase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0008531	riboflavin kinase activity
Process	GO:0009398	FMN biosynthetic process
Process	GO:0009231	riboflavin biosynthetic process
Process	GO:0006771	riboflavin metabolic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	no data	N/A	N/A
Ring	no data	0.26	0.87
Trophozoite	no data	0.33	0.81
Schizont	no data	0.23	0.85
Gametocyte	no data	0.24	0.86

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1359100

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.903 | MFS: -3.001 | #Insertions: 1

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1135400)

Relative Growth Rate: 0.10 ± nan
Confidence: 1.00

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IDB3

"Best" AlphaFill ligand hit: RBF (riboflavine, Local RMSD=1.13) with 1NB9 (Global RMSD=4.50)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.1.26	riboflavin kinase	ATPADPFADFMNNEMPCMBDTNBZnADP-riboflavinlumichromeLumiflavinroseoflavin...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101300

Most Similar Human Ortholog: H7C4G0

TM-align score: 0.82 | RMSD: 1.97

Seq Identity: 0.32 | Length: 114 / 707

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000135002	riboflavin kinase

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	6	37	26	57
disruptive	16	115	81	139
missense	9	101	73	112

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	14	79	32	49
disruptive	35	263	115	191
missense	20	219	88	130

PlasmoDB Total SNPs: 99

Non-coding: 26 | Synonymous: 35 | Nonsynonymous: 38 | Stop Codon: 0

Protein Information

Protein Length: 707 | Molecular Weight (kDa): 83.784

UniProt ID(s): Q8IDB3

PDB ID(s): None

Isoelectric Point: 8.47

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR015865	Riboflavin kinase domain, bacterial/eukaryotic
InterPro	IPR023465	Riboflavin kinase domain superfamily
InterPro	IPR023468	Riboflavin kinase
PFam	PF01687	Riboflavin kinase domain, bacterial/eukaryotic
Superfamily	SSF82114	Riboflavin kinase domain superfamily