Genome location: Pf3D7_13_v3:2,413,324..2,425,261(+)
Genome classification: Core
Product Description: guanylyl cyclase beta
SignalP Peptide: N/A
# Transmembrane Domains: 21
EC Numbers: 4.6.1.2 (Guanylate cyclase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005886 | plasma membrane |
| Function | GO:0004383 | guanylate cyclase activity |
| Process | GO:0071976 | cell gliding |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.06 | 0.97 |
| Trophozoite | possibly expressed | 0.24 | 0.87 |
| Schizont | possibly expressed | 0.05 | 0.97 |
| Gametocyte | expressed | 0.90 | 0.56 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1360500
Resistome Missense Mutations: D2939N, F3060I
Resistome Compounds with Missense Mutations: Desoxyepothilone B, MMV665794
Resistome # Samples with Disruptive Mutations: 6 (4 missense, 4 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -1.392 | #Insertions: 6
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1136700)
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PY17X_1138200)
Modification: Disrupted | RMgm-4795
More info: PhenoPlasm Link
AlphaFill Uniprot ID: N/A
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 4.6.1.2 | guanylate cyclase | BRENDA SOAP query unsuccessful |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 26 | 185 | 93 | 212 |
| disruptive | 101 | 292 | 184 | 392 |
| missense | 78 | 271 | 173 | 347 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 50 | 356 | 150 | 220 |
| disruptive | 162 | 624 | 275 | 636 |
| missense | 120 | 528 | 236 | 489 |
PlasmoDB Total SNPs: 620
Non-coding: 353 | Synonymous: 169 | Nonsynonymous: 98 | Stop Codon: 0
Protein Length: 3179 | Molecular Weight (kDa): 376.99
PDB ID(s): None
Isoelectric Point: 9.77
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001054 | Adenylyl cyclase class-3/4/guanylyl cyclase |
| InterPro | IPR008250 | P-type ATPase, A domain superfamily |
| InterPro | IPR023298 | P-type ATPase, transmembrane domain superfamily |
| InterPro | IPR029787 | Nucleotide cyclase |
| InterPro | IPR032630 | P-type ATPase, C-terminal |
| InterPro | IPR036412 | HAD-like superfamily |
| PFam | PF00211 | Adenylyl cyclase class-3/4/guanylyl cyclase |
| PFam | PF16212 | P-type ATPase, C-terminal |
| Superfamily | SSF55073 | Nucleotide cyclase |
| Superfamily | SSF56784 | HAD-like superfamily |
| Superfamily | SSF81653 | P-type ATPase, A domain superfamily |
| Superfamily | SSF81665 | P-type ATPase, transmembrane domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 10747978 | Guanylyl cyclase activity associated with putative bifunctional integral membraneproteins in Plasmodium falciparum. | Carucci DJ, Witney AA, ..., Baker DA | 10.1074/jbc.M001021200 |
| 11152613 | Predicting transmembrane protein topology with a hidden Markov model: applicationto complete genomes. | Krogh A, Larsson B, von Heijne G, Sonnhammer EL | 10.1006/jmbi.2000.4315 |
| 30146157 | ISP1-Anchored Polarization of GCbeta/CDC50A Complex Initiates Malaria OokineteGliding Motility. | Gao H, Yang Z, ..., Yuan J | 10.1016/j.cub.2018.06.069 |
| 19779564 | A cyclic GMP signalling module that regulates gliding motility in a malariaparasite | Moon RW, Taylor CJ, ..., Billker O | 10.1371/journal.ppat.1000599 |