Genome location: Pf3D7_13_v3:2,425,337..2,432,314(-)
Genome classification: Core
Product Description: E3 SUMO-protein ligase PIAS, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.3.2.27 (RING-type E3 ubiquitin transferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Function | GO:0019789 | SUMO transferase activity |
| Process | GO:0016925 | protein sumoylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 0.40 | 0.80 |
| Trophozoite | expressed | 0.48 | 0.71 |
| Schizont | expressed | 0.27 | 0.82 |
| Gametocyte | expressed | 0.44 | 0.75 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1360700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.124 | MFS: -2.855 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1136900)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: A0A5K1K9G1
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.3.2.27 | RING-type E3 ubiquitin transferase | EDTADTPAMLN4924RAPTA-CRAPTA-TRAPTA-EA1 |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 14 | 78 | 50 | 70 |
| disruptive | 28 | 176 | 83 | 190 |
| missense | 12 | 128 | 67 | 125 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 23 | 153 | 59 | 70 |
| disruptive | 81 | 347 | 143 | 338 |
| missense | 40 | 250 | 100 | 212 |
PlasmoDB Total SNPs: 405
Non-coding: 281 | Synonymous: 81 | Nonsynonymous: 43 | Stop Codon: 0
Protein Length: 1130 | Molecular Weight (kDa): 130.637
UniProt ID(s): A0A5K1K9G1
PDB ID(s): None
Isoelectric Point: 9.2
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001965 | Zinc finger, PHD-type |
| InterPro | IPR004181 | Zinc finger, MIZ-type |
| InterPro | IPR011011 | Zinc finger, FYVE/PHD-type |
| InterPro | IPR019786 | Zinc finger, PHD-type, conserved site |
| InterPro | IPR036361 | SAP domain superfamily |
| PFam | PF02891 | Zinc finger, MIZ-type |
| Superfamily | SSF57903 | Zinc finger, FYVE/PHD-type |
| Superfamily | SSF68906 | SAP domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 23943616 | Identification of biochemically distinct properties of the smallubiquitin-related modifier (SUMO) conjugation pathway in Plasmodium falciparum. | Reiter K, Mukhopadhyay D, ..., Matunis MJ | 10.1074/jbc.M113.498410 |