PF3D7_1361000 (PRMT5)

Genome location: Pf3D7_13_v3:2,444,905..2,447,446(-)

Genome classification: Core

Function and Localization

Product Description: protein arginine N-methyltransferase 5, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.1.1.125 (Transferred entry: 2.1.1.319, 2.1.1.320, 2.1.1.321 and 2.1.1.322);2.1.1.320 (Type II protein arginine methyltransferase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005829	cytosol
Component	GO:0005634	nucleus
Function	GO:0008469	histone-arginine N-methyltransferase activity
Function	GO:0005515	protein binding
Function	GO:0016274	protein-arginine N-methyltransferase activity
Process	GO:0034969	histone arginine methylation
Process	GO:0006355	regulation of transcription, DNA-templated

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.21	0.89
Trophozoite	expressed	0.19	0.89
Schizont	not expressed	0.07	0.95
Gametocyte	not expressed	0.16	0.91

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1361000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: 0.385 | #Insertions: 2

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8ID96

"Best" AlphaFill ligand hit: DXE (1,2-dimethoxyethane, Local RMSD=0.07) with 5IH3 (Global RMSD=6.42)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.1.1.125	histone-arginine N-methyltransferase	BRENDA SOAP query unsuccessful
2.1.1.320	type II protein arginine methyltransferase	GSK3326595dehydrosinefunginS-adenosyl-L-homocysteine(5R)-5'-C-(3,4-difluorophenyl)adenosinemethyl 2-[2-(3,4-dihydroisoquinolin-2(1H)-yl)acetamido]benzoateN'-[3-(3,4-dihydroisoquinolin-2(1H)-yl)propanoyl]benzohydrazidemethyl 2-[4-(3,4-dihydroisoquinolin-2(1H)-yl)butanamido]benzoateN-(2-cyanophenyl)-3-(3,4-dihydroisoquinolin-2(1H)-yl)propanamideN-(3-bromophenyl)-3-(3,4-dihydroisoquinolin-2(1H)-yl)propanamide3-(3,4-dihydroisoquinolin-2(1H)-yl)-N-(3-fluorophenyl)propanamidemethyl 2-[3-(3,4-dihydroisoquinolin-2(1H)-yl)propanamido]benzoateN-(3-acetylphenyl)-3-(3,4-dihydroisoquinolin-2(1H)-yl)propanamide...

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
O14744	Methylosome protein 50/Protein arginine N-methyltransferase 5		HOGENOM, OMA, OrthoDB, OrthoMCL BLAST	P5i-72-((5-(4-methoxyphenyl)furan-2-yl)methylene)hydrazinecarboxamide (4h)2-((4′-chloro-[1,1′-biphenyl]-3-yl)methylene)hydrazinecarboxamide (9b)2-((5-(4-chlorophenyl)pyridin-3-yl)methylene)hydrazinecarboxamide (9f)...

Orthology Information

Ortholog Group (OrthoMCL): OG6_101720

Most Similar Human Ortholog: H0YJD3

TM-align score: 0.93 | RMSD: 1.29

Seq Identity: 0.44 | Length: 172 / 724

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000100462	protein arginine methyltransferase 5

Protein Information

Protein Length: 724 | Molecular Weight (kDa): 85.552

UniProt ID(s): Q8ID96

PDB ID(s): None

Isoelectric Point: 6.83

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR007857	Protein arginine N-methyltransferase PRMT5
InterPro	IPR029063	S-adenosyl-L-methionine-dependent methyltransferase
InterPro	IPR035075	PRMT5 arginine-N-methyltransferase
InterPro	IPR035247	PRMT5, TIM barrel domain
InterPro	IPR035248	PRMT5, oligomerisation domain
PFam	PF05185	PRMT5 arginine-N-methyltransferase
PFam	PF17285	PRMT5, TIM barrel domain
PFam	PF17286	PRMT5, oligomerisation domain
Superfamily	SSF53335	S-adenosyl-L-methionine-dependent methyltransferase

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	6	57	28	56
disruptive	19	101	54	134
missense	13	96	49	122

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	10	112	36	50
disruptive	40	222	86	200
missense	24	197	74	139

PlasmoDB Total SNPs: 65

Non-coding: 11 | Synonymous: 36 | Nonsynonymous: 18 | Stop Codon: 0