Genome location: Pf3D7_13_v3:2,463,755..2,464,745(-)
Genome classification: Core
Product Description: actin-depolymerizing factor 2
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0015629 | actin cytoskeleton |
| Component | GO:0005737 | cytoplasm |
| Function | GO:0051015 | actin filament binding |
| Function | GO:0003785 | actin monomer binding |
| Process | GO:0030036 | actin cytoskeleton organization |
| Process | GO:0030042 | actin filament depolymerization |
| Process | GO:0008154 | actin polymerization or depolymerization |
| Process | GO:0048870 | cell motility |
| Process | GO:0060327 | cytoplasmic actin-based contraction involved in cell motility |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.01 | 0.99 |
| Trophozoite | possibly expressed | 0.01 | 0.99 |
| Schizont | expressed | 0.12 | 0.92 |
| Gametocyte | expressed | 0.17 | 0.89 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1361400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: 0.697 | #Insertions: 6
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1103100)
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_1137500)
Modification: Disrupted | RMgm-401
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8ID92
"Best" AlphaFill ligand hit: TAR (d(-)-tartaric acid, Local RMSD=3.29) with 3Q2B (Global RMSD=4.09)
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100500
Most Similar Human Ortholog: P23528
TM-align score: 0.87 | RMSD: 1.73
Seq Identity: 0.31 | Length: 137 / 143
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000125868 | destrin, actin depolymerizing factor |
| ENSG00000165410 | cofilin 2 |
| ENSG00000172757 | cofilin 1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 12 | 5 | 8 |
| disruptive | 0 | 4 | 4 | 9 |
| missense | 0 | 4 | 4 | 7 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 14 | 9 | 13 |
| disruptive | 0 | 14 | 11 | 21 |
| missense | 0 | 11 | 8 | 15 |
PlasmoDB Total SNPs: 42
Non-coding: 39 | Synonymous: 0 | Nonsynonymous: 3 | Stop Codon: 0
Protein Length: 143 | Molecular Weight (kDa): 16.998
UniProt ID(s): Q8ID92
PDB ID(s): None
Isoelectric Point: 5.34
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002108 | Actin-depolymerising factor homology domain |
| InterPro | IPR017904 | ADF/Cofilin |
| PFam | PF00241 | Actin-depolymerising factor homology domain |
| Superfamily | SSF55753 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 15975905 | A Plasmodium actin-depolymerizing factor that binds exclusively to actinmonomers. | Schuler H, Mueller AK, Matuschewski K | 10.1091/mbc.e05-02-0086 |
| 21628589 | Minimal requirements for actin filament disassembly revealed by structuralanalysis of malaria parasite actin-depolymerizing factor 1. | Wong W, Skau CT, ..., Baum J | 10.1073/pnas.1018927108 |
| 24371134 | A mechanism for actin filament severing by malaria parasite actin depolymerizingfactor 1 via a low affinity binding interface. | Wong W, Webb AI, ..., Baum J | 10.1074/jbc.M113.523365 |
| 20529666 | ADF2 is required for transformation of the ookinete and sporozoite in malariaparasite development | Doi Y, Shinzawa N, ..., Kanuka H | 10.1016/j.bbrc.2010.05.155 |
| 3305160 | [Mutants of Saccharomyces cerevisiae characterized by increased level of inducedmutagenesis. I. Isolation and preliminary characterization of mutants] | Ivanov EL, Koval'tsova SV, Korolev VG | https://pubmed.ncbi.nlm.nih.gov/3305160/ |
| 21832095 | Crystal structures explain functional differences in the two actindepolymerization factors of the malaria parasite | Singh BK, Sattler JM, ..., Kursula I | 10.1074/jbc.M111.211730 |