Genome location: Pf3D7_13_v3:2,481,776..2,484,101(-)
Genome classification: Core
Product Description: proliferating cell nuclear antigen 1
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0043626 | PCNA complex |
| Component | GO:0005829 | cytosol |
| Component | GO:0005634 | nucleus |
| Function | GO:0030337 | DNA polymerase processivity factor activity |
| Process | GO:0006272 | leading strand elongation |
| Process | GO:0006298 | mismatch repair |
| Process | GO:0019985 | translesion synthesis |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.16 | 0.93 |
| Trophozoite | expressed | 1.30 | 0.38 |
| Schizont | expressed | 0.48 | 0.72 |
| Gametocyte | expressed | 0.52 | 0.73 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1361900
Resistome Missense Mutations: N215K
Resistome Compounds with Missense Mutations: AN10248, AN13762
Resistome # Samples with Disruptive Mutations: 5 (5 missense, 5 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.234 | MFS: -3.274 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: P61074
"Best" AlphaFill ligand hit: TME (propane, Local RMSD=0.08) with 7M5L (Global RMSD=1.65)
No associated EC numbersOrthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P12004 | Proliferating cell nuclear antigen | Homo sapiens | HOGENOM, OMA, OrthoDB | CHEMBL2348305CHEMBL2348283CHEMBL2348282CHEMBL2348304... |
Ortholog Group (OrthoMCL): OG6_100882
Most Similar Human Ortholog: P12004
TM-align score: 0.96 | RMSD: 1.62
Seq Identity: 0.36 | Length: 260 / 274
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000132646 | proliferating cell nuclear antigen |
Protein Length: 274 | Molecular Weight (kDa): 30.587
UniProt ID(s): A0A5K1K8U9, P61074
PDB ID(s): None
Isoelectric Point: 4.44
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000730 | Proliferating cell nuclear antigen, PCNA |
| InterPro | IPR022648 | Proliferating cell nuclear antigen, PCNA, N-terminal |
| InterPro | IPR022649 | Proliferating cell nuclear antigen, PCNA, C-terminal |
| InterPro | IPR022659 | Proliferating cell nuclear antigen, PCNA, conserved site |
| PFam | PF00705 | Proliferating cell nuclear antigen, PCNA, N-terminal |
| PFam | PF02747 | Proliferating cell nuclear antigen, PCNA, C-terminal |
| Superfamily | SSF55979 | N/A |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 11 | 5 | 7 |
| disruptive | 0 | 4 | 3 | 2 |
| missense | 0 | 4 | 2 | 0 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 24 | 6 | 17 |
| disruptive | 1 | 13 | 3 | 18 |
| missense | 0 | 9 | 2 | 13 |
PlasmoDB Total SNPs: 68
Non-coding: 63 | Synonymous: 4 | Nonsynonymous: 0 | Stop Codon: 1
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 19633266 | Functional dissection of the catalytic carboxyl-terminal domain of originrecognition complex subunit 1 (PfORC1) of the human malaria parasite Plasmodiumfalciparum. | Gupta A, Mehra P, ..., Dhar SK | 10.1128/EC.00170-09 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 35353560 | Asynchronous nuclear cycles in multinucleated Plasmodium falciparum facilitaterapid proliferation. | Klaus S, Binder P, ..., Ganter M | 10.1126/sciadv.abj5362 |
| 34374200 | Uptake of hematite nanoparticles in maize and their role in cell cycle dynamics,PCNA expression and mitigation of cadmium stress | Youssef OA, Tammam AA, ..., El-Sadek LM | 10.1111/plb.13315 |
| 30865364 | Molecular characterization and expression profile of an alternate proliferatingcell nuclear antigen homolog PbPCNA2 in Plasmodium berghei | Pradhan S, Kalia I, ..., Dhar SK | 10.1002/iub.2036 |
| 30052905 | Role of tyrosine residue (Y213) in nuclear retention of PCNA1 in human malariaparasite Plasmodium falciparum | Banu K, Mitra P, Subbarao N, Dhar SK | 10.1093/femsle/fny182 |
| 26893139 | An external sensing system in Plasmodium falciparum-infected erythrocytes | Wu Y, Cruz LN, ..., Craig AG | 10.1186/s12936-016-1144-6 |