PF3D7_1362100 (14-3-3II)

Genome location: Pf3D7_13_v3:2,487,843..2,489,579(-)

Genome classification: Core

Function and Localization

Product Description: 14-3-3 protein

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	not expressed	0.05	0.98
Trophozoite	not expressed	0.01	0.99
Schizont	not expressed	0.07	0.96
Gametocyte	expressed	0.61	0.68

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1362100

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.666 | MFS: -2.163 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1138100)

Relative Growth Rate: 1.04 ± 0.09
Confidence: 6.24

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1138100)

Modification: Disrupted | RMgm-3125

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: A0A5K1K948

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_179356

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	1	19	18	17
disruptive	8	12	10	25
missense	8	12	10	20

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	3	44	12	25
disruptive	10	31	25	56
missense	10	26	24	38

PlasmoDB Total SNPs: 122

Non-coding: 93 | Synonymous: 19 | Nonsynonymous: 8 | Stop Codon: 2

Protein Information

Protein Length: 273 | Molecular Weight (kDa): 31.776

UniProt ID(s): A0A5K1K948

PDB ID(s): None

Isoelectric Point: 7.42

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000308	14-3-3 protein
InterPro	IPR023410	14-3-3 domain
InterPro	IPR036815	14-3-3 domain superfamily
PFam	PF00244	14-3-3 domain
Superfamily	SSF48445	14-3-3 domain superfamily