Genome location: Pf3D7_13_v3:2,489,913..2,492,561(-)
Genome classification: Core
Product Description: RuvB-like helicase 3
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.4.12 (DNA helicase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0031011 | Ino80 complex |
| Component | GO:0035267 | NuA4 histone acetyltransferase complex |
| Component | GO:0097255 | R2TP complex |
| Component | GO:0000812 | Swr1 complex |
| Component | GO:0044538 | host cell periphery |
| Component | GO:0005634 | nucleus |
| Component | GO:0020003 | symbiont-containing vacuole |
| Function | GO:0016887 | ATP hydrolysis activity |
| Function | GO:0003678 | DNA helicase activity |
| Function | GO:0005515 | protein binding |
| Process | GO:0000492 | box C/D snoRNP assembly |
| Process | GO:0006338 | chromatin remodeling |
| Process | GO:0016573 | histone acetylation |
| Process | GO:0006357 | regulation of transcription by RNA polymerase II |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.24 | 0.88 |
| Trophozoite | expressed | 0.38 | 0.78 |
| Schizont | expressed | 0.05 | 0.97 |
| Gametocyte | not expressed | 0.38 | 0.78 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1362200
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -2.266 | #Insertions: 1
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1138200)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8ID85
"Best" AlphaFill ligand hit: 3AT (cordycepin triphosphate, Local RMSD=0.13) with 6K0R (Global RMSD=1.80)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.4.12 | DNA helicase | No BRENDA inhibitors |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q9Y265 | RuvB-like 1 | OrthoDB | CHEMBL3263107CHEMBL5178717CHEMBL3263107 |
Ortholog Group (OrthoMCL): OG6_101635
Most Similar Human Ortholog: Q9Y230
TM-align score: 0.94 | RMSD: 1.83
Seq Identity: 0.68 | Length: 455 / 483
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000183207 | RuvB like AAA ATPase 2 |
Protein Length: 483 | Molecular Weight (kDa): 54.629
UniProt ID(s): Q8ID85
PDB ID(s): None
Isoelectric Point: 5.12
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR003593 | AAA+ ATPase domain |
| InterPro | IPR010339 | TIP49, P-loop domain |
| InterPro | IPR027238 | RuvB-like |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR037942 | RuvB-like helicase 2 |
| InterPro | IPR041048 | RuvB-like, AAA-lid domain |
| PFam | PF06068 | TIP49, P-loop domain |
| PFam | PF17856 | RuvB-like, AAA-lid domain |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 34 | 13 | 24 |
| disruptive | 2 | 12 | 12 | 47 |
| missense | 2 | 12 | 11 | 41 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 12 | 55 | 12 | 23 |
| disruptive | 6 | 43 | 33 | 79 |
| missense | 5 | 37 | 25 | 62 |
PlasmoDB Total SNPs: 120
Non-coding: 103 | Synonymous: 11 | Nonsynonymous: 6 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 22705314 | Novel RuvB nuclear ATPase is specific to intraerythrocytic mitosis duringschizogony of Plasmodium falciparum. | Ahmad M, Singh S, Afrin F, Tuteja R | https://pubmed.ncbi.nlm.nih.gov/22705314/ |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 24161468 | Plasmodium falciparum RuvB2 translocates in 5'-3' direction, relocalizes duringschizont stage and its enzymatic activities are up regulated by RuvB3 of the samecomplex. | Ahmad M, Tuteja R | 10.1016/j.bbapap.2013.10.010 |
| 27809838 | Characterization of Plasmodium falciparum ATP-dependent DNA helicase RuvB3. | Limudomporn P, Moonsom S, ..., Chavalitshewinkoon-Petmitr P | 10.1186/s12936-016-1573-2 |
| 30026605 | Plasmodium falciparum RUVBL3 protein: a novel DNA modifying enzyme and aninteracting partner of essential HAT protein MYST. | Sen U, Saxena H, ..., Gupta A | 10.1038/s41598-018-29137-8 |