Genome location: Pf3D7_13_v3:2,495,001..2,502,249(+)
Genome classification: Core
Product Description: calpain
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.4.22.- (Cysteine endopeptidases.)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005730 | nucleolus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.47 | 0.78 |
| Trophozoite | possibly expressed | 0.54 | 0.69 |
| Schizont | possibly expressed | 0.59 | 0.66 |
| Gametocyte | expressed | 1.07 | 0.47 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1362400
Resistome Missense Mutations: N443K
Resistome Compounds with Missense Mutations: AN13762
Resistome # Samples with Disruptive Mutations: 3 (2 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.245 | MFS: -2.707 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1138400)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: A0A5K1K8Q4
"Best" AlphaFill ligand hit: E64 (n-[n-[1-hydroxycarboxyethyl-carbonyl]leucylamino-butyl]-guanidine, Local RMSD=0.26) with 6BJD (Global RMSD=3.45)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.4.22.- | Ex: staphylococcal cysteine proteinase | NEME64SpiSDSCysEDTAPCMBEGTAPMSFTLCKTPCKMENT... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_103851
Most Similar Human Ortholog: Q9Y6W3
TM-align score: 0.68 | RMSD: 3.88
Seq Identity: 0.29 | Length: 612 / 2048
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000131375 | calpain 7 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 20 | 141 | 74 | 151 |
| disruptive | 100 | 326 | 159 | 390 |
| missense | 60 | 272 | 140 | 333 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 42 | 280 | 100 | 139 |
| disruptive | 184 | 657 | 301 | 627 |
| missense | 106 | 537 | 236 | 372 |
PlasmoDB Total SNPs: 350
Non-coding: 103 | Synonymous: 133 | Nonsynonymous: 113 | Stop Codon: 1
Protein Length: 2048 | Molecular Weight (kDa): 242.552
UniProt ID(s): A0A5K1K8Q4, B9P4K9, E5LAD0
PDB ID(s): None
Isoelectric Point: 9.08
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001300 | Peptidase C2, calpain, catalytic domain |
| InterPro | IPR036213 | Calpain large subunit, domain III superfamily |
| InterPro | IPR038765 | Papain-like cysteine peptidase superfamily |
| PFam | PF00648 | Peptidase C2, calpain, catalytic domain |
| Superfamily | SSF49758 | Calpain large subunit, domain III superfamily |
| Superfamily | SSF54001 | Papain-like cysteine peptidase superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 19164769 | A calpain unique to alveolates is essential in Plasmodium falciparum and itsknockdown reveals an involvement in pre-S-phase development. | Russo I, Oksman A, Vaupel B, Goldberg DE | 10.1073/pnas.0806926106 |
| 19239622 | Fatty acid acylation regulates trafficking of the unusual Plasmodium falciparumcalpain to the nucleolus. | Russo I, Oksman A, Goldberg DE | 10.1111/j.1365-2958.2009.06639.x |
| 19861123 | Confocal microscopic findings of cysteine protease calpain in Plasmodiumfalciparum. | Choi YY, Jung SY, ..., Park H | 10.1016/j.exppara.2009.10.007 |