Genome location: Pf3D7_13_v3:2,502,730..2,505,875(+)
Genome classification: Core
Product Description: 3'-5' exonuclease, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.1.-.- (Acting on ester bonds.)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005739 | mitochondrion |
| Process | GO:0009410 | response to xenobiotic stimulus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | not expressed | 0.04 | 0.98 |
| Trophozoite | possibly expressed | 0.23 | 0.85 |
| Schizont | not expressed | 0.18 | 0.88 |
| Gametocyte | possibly expressed | 0.26 | 0.85 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1362500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.165 | MFS: -3.267 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1138500)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: A0A5K1K919
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.-.- | Ex: 5-phospho-D-xylono-1,4-lactonase | paraoxonatropineethylene terephthalatediisopropyl fluorophosphonatebis(2-hydroxyethyl) terephthalic acidphosphoric acid-bis-4-nitrophenyl ester |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 34 | 28 | 53 |
| disruptive | 19 | 100 | 51 | 132 |
| missense | 15 | 91 | 46 | 116 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 79 | 29 | 58 |
| disruptive | 47 | 199 | 87 | 170 |
| missense | 32 | 168 | 73 | 135 |
PlasmoDB Total SNPs: 181
Non-coding: 125 | Synonymous: 37 | Nonsynonymous: 18 | Stop Codon: 1
Protein Length: 713 | Molecular Weight (kDa): 86.675
UniProt ID(s): A0A249JQ57, A0A249JQ63, A0A249JQ66, A0A249JQ72, A0A249JQ96, A0A249JQB3, A0A249JQH6, A0A291PWN9, A0A291PWQ6, A0A291PWR9, A0A291PWU8, A0A5K1K919
PDB ID(s): None
Isoelectric Point: 9.3
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002562 | 3'-5' exonuclease domain |
| InterPro | IPR012337 | Ribonuclease H-like superfamily |
| PFam | PF01612 | 3'-5' exonuclease domain |
| Superfamily | SSF53098 | Ribonuclease H-like superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 8944766 | Computational method to predict mitochondrially imported proteins and theirtargeting sequences. | Claros MG, Vincens P | 10.1111/j.1432-1033.1996.00779.x |
| 27818095 | Genetic markers associated with dihydroartemisinin-piperaquine failure inPlasmodium falciparum malaria in Cambodia: a genotype-phenotype associationstudy. | Amato R, Lim P, ..., Fairhurst RM | 10.1016/S1473-3099(16)30409-1 |