Genome location: Pf3D7_13_v3:2,547,654..2,551,108(+)
Genome classification: Core
Product Description: CCR4 domain-containing protein 2, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.1.21.1 (Deoxyribonuclease I)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005739 | mitochondrion |
| Component | GO:0005634 | nucleus |
| Function | GO:0004518 | nuclease activity |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.12 | 0.94 |
| Trophozoite | expressed | 0.61 | 0.65 |
| Schizont | expressed | 0.56 | 0.67 |
| Gametocyte | possibly expressed | 0.79 | 0.57 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1363500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -0.314 | #Insertions: 11
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1139500)
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PY17X_1140900)
Modification: Disrupted | RMgm-4585
More info: PhenoPlasm Link
AlphaFill Uniprot ID: A0A5K1K929
"Best" AlphaFill ligand hit: ADN (adenosine, Local RMSD=0.04) with 6RVZ (Global RMSD=12.05)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.21.1 | deoxyribonuclease I | KClSDSDTTRNAEDTAEGTAactin3'-AMP5'-GMPHPO42-P2O74-5'-GTP... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 10 | 55 | 13 | 57 |
| disruptive | 34 | 180 | 84 | 174 |
| missense | 28 | 152 | 74 | 142 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 20 | 96 | 36 | 64 |
| disruptive | 75 | 332 | 122 | 276 |
| missense | 54 | 261 | 94 | 169 |
PlasmoDB Total SNPs: 158
Non-coding: 76 | Synonymous: 41 | Nonsynonymous: 39 | Stop Codon: 2
Protein Length: 836 | Molecular Weight (kDa): 99.889
UniProt ID(s): A0A5K1K929
PDB ID(s): None
Isoelectric Point: 8.93
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR036691 | Endonuclease/exonuclease/phosphatase superfamily |
| PFam | N/A | N/A |
| Superfamily | SSF56219 | Endonuclease/exonuclease/phosphatase superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 29914511 | Identification and characterization of DNA endonucleases in Plasmodium falciparum3D7 clone. | Jiang N, Tu Z, ..., Chen Q | 10.1186/s12936-018-2388-0 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |