Genome location: Pf3D7_13_v3:2,552,035..2,555,363(+)
Genome classification: Core
Product Description: protein AMR1
SignalP Peptide: N/A
# Transmembrane Domains: 1
EC Numbers: 4.1.1.48 (Indole-3-glycerol-phosphate synthase);4.1.3.27 (Anthranilate synthase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0009986 | cell surface |
| Function | GO:0004425 | indole-3-glycerol-phosphate synthase activity |
| Process | GO:0009657 | plastid organization |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.05 | 0.97 |
| Trophozoite | possibly expressed | 0.42 | 0.75 |
| Schizont | possibly expressed | 0.13 | 0.92 |
| Gametocyte | not expressed | 0.28 | 0.84 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1363700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.194 | MFS: -2.633 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1139700)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: A0A5K1K8R3
"Best" AlphaFill ligand hit: 137 (1-(o-carboxy-phenylamino)-1-deoxy-d-ribulose-5-phosphate, Local RMSD=0.69) with 6Y88 (Global RMSD=3.67)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 4.1.1.48 | indole-3-glycerol-phosphate synthase | ATB107methanolphosphatekanamycingeomycinetryptophanL-tryptophanstreptomycinReduced 1-[2-carboxyphenyl)amino]-1-deoxyribulose 5-phosphate |
| 4.1.3.27 | anthranilate synthase | NEMGluGlnPCMBMnCl2L-TrppyruvatetryptaminetryptophanmethylamineL-tryptophananthranilate... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 43 | 24 | 62 |
| disruptive | 20 | 122 | 59 | 149 |
| missense | 11 | 110 | 56 | 133 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 10 | 93 | 39 | 62 |
| disruptive | 36 | 228 | 81 | 224 |
| missense | 19 | 203 | 75 | 160 |
PlasmoDB Total SNPs: 164
Non-coding: 96 | Synonymous: 35 | Nonsynonymous: 33 | Stop Codon: 0
Protein Length: 790 | Molecular Weight (kDa): 92.786
UniProt ID(s): A0A5K1K8R3
PDB ID(s): None
Isoelectric Point: 6.32
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR011060 | Ribulose-phosphate binding barrel |
| InterPro | IPR013798 | Indole-3-glycerol phosphate synthase |
| PFam | PF00218 | Indole-3-glycerol phosphate synthase |
| Superfamily | SSF51366 | Ribulose-phosphate binding barrel |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 23418676 | Expression profile of the Plasmodium falciparum intra-erythrocytic stage protein,PF3D7_1363700. | Roberts RN, Schlarman MS, ..., Beerntsen BT | 10.1186/1475-2875-12-66 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 30726238 | A mutagenesis screen for essential plastid biogenesis genes in human malariaparasites. | Tang Y, Meister TR, ..., Yeh E | 10.1371/journal.pbio.3000136 |
| 31648168 | In-silico structural modeling and epitope prediction of highly conserved Plasmodium falciparum protein AMR1 | Sanasam BD, Kumar S | 10.1016/j.molimm.2019.10.003 |