Genome location: Pf3D7_13_v3:2,556,025..2,559,547(+)
Genome classification: Core
Product Description: nuclear fusion protein, putative
SignalP Peptide: MLNLFVFLILFIIRFIVIRG
# Transmembrane Domains: 4
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005635 | nuclear envelope |
| Process | GO:0000741 | karyogamy |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.01 | 0.99 |
| Trophozoite | not expressed | 0.07 | 0.95 |
| Schizont | possibly expressed | 0.04 | 0.98 |
| Gametocyte | expressed | 0.58 | 0.68 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1363800
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: 0.055 | #Insertions: 2
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1139800)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1139800)
Modification: Disrupted | RMgm-3136
More info: PhenoPlasm Link
AlphaFill Uniprot ID: A0A5K1K926
"Best" AlphaFill ligand hit: U5P (uridine-5'-monophosphate, Local RMSD=0.54) with 6DSR (Global RMSD=17.47)
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 36 | 25 | 42 |
| disruptive | 10 | 54 | 41 | 88 |
| missense | 10 | 54 | 39 | 82 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 11 | 73 | 40 | 31 |
| disruptive | 25 | 131 | 73 | 136 |
| missense | 21 | 115 | 67 | 102 |
PlasmoDB Total SNPs: 192
Non-coding: 158 | Synonymous: 24 | Nonsynonymous: 10 | Stop Codon: 0
Protein Length: 681 | Molecular Weight (kDa): 81.402
UniProt ID(s): A0A5K1K926
PDB ID(s): None
Isoelectric Point: 6.39
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR040346 | GEX1/Brambleberry |
| PFam | N/A | N/A |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 23699412 | Comparative genomics in Chlamydomonas and Plasmodium identifies an ancientnuclear envelope protein family essential for sexual reproduction in protists,fungi, plants, and vertebrates. | Ning J, Otto TD, ..., Snell WJ | 10.1101/gad.212746.112 |
| 25467943 | Kar5p is required for multiple functions in both inner and outer nuclear envelopefusion in Saccharomyces cerevisiae | Rogers JV, Rose MD | 10.1534/g3.114.015800 |
| 33546197 | Screening and Genetic Network Analysis of Genes Involved in Freezing and ThawingResistance in DaMDHAR-Expressing Saccharomyces cerevisiae Using Gene ExpressionProfiling | Kim IS, Choi W, ..., Kim HW | 10.3390/genes12020219 |
| 21490148 | Gex1 is a yeast glutathione exchanger that interferes with pH and redoxhomeostasis | Dhaoui M, Auchere F, ..., Belgareh-Touze N | 10.1091/mbc.E10-11-0906 |