Genome location: Pf3D7_13_v3:2,596,245..2,598,309(+)
Genome classification: Core
Product Description: exosome complex component RRP45, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.7.56 (tRNA nucleotidyltransferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0000177 | cytoplasmic exosome (RNase complex) |
| Component | GO:0000178 | exosome (RNase complex) |
| Component | GO:0000176 | nuclear exosome (RNase complex) |
| Function | GO:0000175 | 3'-5'-exoribonuclease activity |
| Process | GO:0006396 | RNA processing |
| Process | GO:0034473 | U1 snRNA 3'-end processing |
| Process | GO:0034475 | U4 snRNA 3'-end processing |
| Process | GO:0034476 | U5 snRNA 3'-end processing |
| Process | GO:0043928 | exonucleolytic catabolism of deadenylated mRNA |
| Process | GO:0000467 | exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) |
| Process | GO:0071028 | nuclear mRNA surveillance |
| Process | GO:0071042 | nu |
| Process | GO:0071035 | |
| Process | GO:0071038 | |
| Process | GO:0034427 | |
| Process | GO:0016075 |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.25 | 0.87 |
| Trophozoite | possibly expressed | 0.36 | 0.78 |
| Schizont | expressed | 0.05 | 0.96 |
| Gametocyte | possibly expressed | 0.26 | 0.84 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1364500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.161 | MFS: -2.735 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8ID62
"Best" AlphaFill ligand hit: U5P (uridine-5'-monophosphate, Local RMSD=0.01) with 2C37 (Global RMSD=2.67)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.7.56 | tRNA nucleotidyltransferase | KClEDTAphosphateN-ethylmaleimidep-hydroxymercuribenzoate |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100420
Most Similar Human Ortholog: D6RA17
TM-align score: 0.92 | RMSD: 1.70
Seq Identity: 0.31 | Length: 236 / 477
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000120699 | exosome component 8 |
| ENSG00000123737 | exosome component 9 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 33 | 16 | 32 |
| disruptive | 11 | 69 | 45 | 82 |
| missense | 10 | 65 | 44 | 72 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 10 | 53 | 20 | 46 |
| disruptive | 27 | 135 | 59 | 126 |
| missense | 20 | 127 | 57 | 90 |
PlasmoDB Total SNPs: 90
Non-coding: 56 | Synonymous: 20 | Nonsynonymous: 14 | Stop Codon: 0
Protein Length: 477 | Molecular Weight (kDa): 55.027
UniProt ID(s): Q8ID62
PDB ID(s): None
Isoelectric Point: 5.66
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001247 | Exoribonuclease, phosphorolytic domain 1 |
| InterPro | IPR015847 | Exoribonuclease, phosphorolytic domain 2 |
| InterPro | IPR020568 | Ribosomal protein S5 domain 2-type fold |
| InterPro | IPR033100 | Exosome complex component RRP45 |
| InterPro | IPR036345 | Exoribonuclease, PH domain 2 superfamily |
| PFam | PF01138 | Exoribonuclease, phosphorolytic domain 1 |
| PFam | PF03725 | Exoribonuclease, phosphorolytic domain 2 |
| Superfamily | SSF54211 | Ribosomal protein S5 domain 2-type fold |
| Superfamily | SSF55666 | Exoribonuclease, PH domain 2 superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 30235972 | Disruption of the RNA exosome reveals the hidden face of the malaria parasitetranscriptome. | Droll D, Wei G, ..., Zhang Q | 10.1080/15476286.2018.1517014 |
| 24105744 | A SUMO-dependent interaction between Senataxin and the exosome, disrupted in theneurodegenerative disease AOA2, targets the exosome to sites oftranscription-induced DNA damage | Richard P, Feng S, Manley JL | 10.1101/gad.224923.113 |
| 14624005 | A role for the exosome in the in vivo degradation of unstable mRNAs | Haile S, Estevez AM, Clayton C | 10.1261/rna.5940703 |
| 17187872 | Regulated expression of glycosomal phosphoglycerate kinase in Trypanosoma brucei | Colasante C, Robles A, ..., Clayton C | 10.1016/j.molbiopara.2006.11.003 |
| 23697549 | The roles of 3'-exoribonucleases and the exosome in trypanosome mRNA degradation | Fadda A, Farber V, Droll D, Clayton C | 10.1261/rna.038430.113 |
| 19879841 | The yeast exosome functions as a macromolecular cage to channel RNA substratesfor degradation | Bonneau F, Basquin J, ..., Conti E | 10.1016/j.cell.2009.08.042 |
| 17174896 | Reconstitution, activities, and structure of the eukaryotic RNA exosome | Liu Q, Greimann JC, Lima CD | 10.1016/j.cell.2006.10.037 |
| 17942686 | Architecture of the yeast Rrp44 exosome complex suggests routes of RNArecruitment for 3' end processing | Wang HW, Wang J, ..., Ke A | 10.1073/pnas.0705526104 |
| 19327365 | Crystal structure of Escherichia coli polynucleotide phosphorylase core bound toRNase E, RNA and manganese: implications for catalytic mechanism and RNAdegradosome assembly | Nurmohamed S, Vaidialingam B, Callaghan AJ, Luisi BF | 10.1016/j.jmb.2009.03.051 |
| 31768969 | The RNA Exosome and Human Disease | Fasken MB, Morton DJ, ..., Corbett AH | 10.1007/978-1-4939-9822-7_1 |