Genome location: Pf3D7_13_v3:2,603,194..2,605,638(-)
Genome classification: Core
Product Description: DNA-directed RNA polymerases I, II, and III subunit RPABC1, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.7.6 (DNA-directed RNA polymerase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005736 | RNA polymerase I complex |
| Component | GO:0005665 | RNA polymerase II, core complex |
| Component | GO:0005666 | RNA polymerase III complex |
| Component | GO:0005634 | nucleus |
| Function | GO:0003677 | DNA binding |
| Function | GO:0003899 | DNA-directed 5'-3' RNA polymerase activity |
| Process | GO:0006366 | transcription by RNA polymerase II |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.26 | 0.87 |
| Trophozoite | expressed | 0.20 | 0.88 |
| Schizont | expressed | 0.02 | 0.99 |
| Gametocyte | expressed | 0.17 | 0.89 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1364800
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.224 | MFS: -2.651 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1140600)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8ID59
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.7.6 | DNA-directed RNA polymerase | BRENDA SOAP query unsuccessful |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101049
Most Similar Human Ortholog: P19388
TM-align score: 0.95 | RMSD: 1.58
Seq Identity: 0.47 | Length: 204 / 205
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000099817 | RNA polymerase II, I and III subunit E |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 0 | 0 | 0 |
| disruptive | 0 | 0 | 0 | 0 |
| missense | 0 | 0 | 0 | 0 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 0 | 0 | 0 |
| disruptive | 0 | 0 | 0 | 1 |
| missense | 0 | 0 | 0 | 0 |
PlasmoDB Total SNPs: 203
Non-coding: 201 | Synonymous: 2 | Nonsynonymous: 0 | Stop Codon: 0
Protein Length: 205 | Molecular Weight (kDa): 24.154
UniProt ID(s): Q8ID59
PDB ID(s): None
Isoelectric Point: 9.52
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000783 | RNA polymerase, subunit H/Rpb5 C-terminal |
| InterPro | IPR005571 | RNA polymerase, Rpb5, N-terminal |
| InterPro | IPR014381 | DNA-directed RNA polymerase RPB5 subunit, eukaryote/virus |
| InterPro | IPR020609 | Archaeal RpoH /eukaryotic RPB5 RNA polymerase subunit |
| InterPro | IPR035913 | RPB5-like RNA polymerase subunit superfamily |
| InterPro | IPR036710 | RNA polymerase Rpb5, N-terminal domain superfamily |
| InterPro | IPR039531 | DNA-directed RNA polymerase subunit Rpb5-like |
| PFam | PF01191 | RNA polymerase, subunit H/Rpb5 C-terminal |
| PFam | PF03871 | RNA polymerase, Rpb5, N-terminal |
| Superfamily | SSF53036 | RNA polymerase Rpb5, N-terminal domain superfamily |
| Superfamily | SSF55287 | RPB5-like RNA polymerase subunit superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 34161395 | Novel aspects of iron homeostasis in pathogenic bloodstream form Trypanosomabrucei | Gilabert Carbajo C, Cornell LJ, ..., Tiengwe C | 10.1371/journal.ppat.1009696 |
| 32503921 | Prefoldin-like Bud27 influences the transcription of ribosomal components andribosome biogenesis in Saccharomyces cerevisiae | Martinez-Fernandez V, Cuevas-Bermudez A, ..., Navarro F | 10.1261/rna.075507.120 |
| 37967810 | The association of the RSC remodeler complex with chromatin is influenced by theprefoldin-like Bud27 and determines nucleosome positioning and polyadenylationsites usage in Saccharomyces cerevisiae | Cuevas-Bermudez A, Martinez-Fernandez V, ..., Navarro F | 10.1016/j.bbagrm.2023.194995 |