Product Description: ferrochelatase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 4.99.1.1 (Protoporphyrin ferrochelatase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005739 | mitochondrion |
| Function | GO:0004325 | ferrochelatase activity |
| Process | GO:0006783 | heme biosynthetic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.02 | 0.99 |
| Trophozoite | possibly expressed | 0.08 | 0.95 |
| Schizont | possibly expressed | 0.01 | 0.99 |
| Gametocyte | not expressed | 0.04 | 0.98 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1364900
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -0.852 | #Insertions: 1
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1140700)
Modification: Disrupted | RMgm-5263
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8ID58
"Best" AlphaFill ligand hit: CHD (cholic acid, Local RMSD=0.45) with 3W1W (Global RMSD=4.30)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 4.99.1.1 | protoporphyrin ferrochelatase | No BRENDA inhibitors |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P22830 | Ferrochelatase, mitochondrial | OrthoDB | VEMURAFENIB |
Ortholog Group (OrthoMCL): OG6_101310
Most Similar Human Ortholog: P22830
TM-align score: 0.84 | RMSD: 2.42
Seq Identity: 0.26 | Length: 320 / 350
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000066926 | ferrochelatase |
Protein Length: 350 | Molecular Weight (kDa): 40.81
UniProt ID(s): Q8ID58, Q8IFQ9, Q8IFR0
PDB ID(s): None
Isoelectric Point: 9.7
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001015 | Ferrochelatase |
| InterPro | IPR019772 | Ferrochelatase, active site |
| InterPro | IPR033644 | Ferrochelatase, C-terminal |
| InterPro | IPR033659 | Ferrochelatase, N-terminal |
| PFam | PF00762 | Ferrochelatase |
| Superfamily | SSF53800 | N/A |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 30 | 10 | 29 |
| disruptive | 3 | 23 | 14 | 41 |
| missense | 3 | 23 | 13 | 40 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 10 | 46 | 10 | 35 |
| disruptive | 9 | 48 | 28 | 75 |
| missense | 8 | 43 | 24 | 56 |
PlasmoDB Total SNPs: 131
Non-coding: 115 | Synonymous: 11 | Nonsynonymous: 5 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 19523497 | Mitochondrial localization of functional ferrochelatase from Plasmodiumfalciparum. | Nagaraj VA, Prasad D, Rangarajan PN, Padmanaban G | 10.1016/j.molbiopara.2009.05.008 |
| 25352601 | The heme biosynthesis pathway is essential for Plasmodium falciparum developmentin mosquito stage but not in blood stages. | Ke H, Sigala PA, ..., Vaidya AB | 10.1074/jbc.M114.615831 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |